Dna Replication Research Paper

DNA replication is an intricate process that requires many different proteins. Each protein preforms a very specific function in the creation of a new DNA strand. First helicase works by unwinding or dividing the original double helix into single stands. The point where the DNA is separated by the helicase is known as the replication fork. Single strand binding proteins attach to the newly made single strand of DNA to prevent re-annealing. Next is the addition of an RNA

The structure of DNA is compared to that of a spiral ladder. A complementary base pair, held together by a hydrogen bond, creates the “rungs” of the ladder. The sugar and phosphate together create the backbone, or the sides of the ladder. The carbon atoms of the free sugar are numbered 1-5, and the sugar contains two “ends” that contribute to the synthesizing of the backbone: the 3’ and 5’ ends which connect to the phosphate group of the adjacent nucleotide. And now since there are the ends, there will always be one 3’ end not attached to a phosphate group and a phosphate group not attached to a 3’ end at the end of the backbone. The sugar-phosphate backbones of the two strands of a double helix are antiparallel, meaning that they run in different directions.

DNA is the genetic material of cells and the production of RNA and/or proteins from the information stored in DNA is called gene expression. There are two steps in gene expression, transcription and translation.

Each human being has something called DNA. DNA is described as genetics and an extremely long macromolecule that is the main component of chromosomes and is the material that transfers genetic characteristics in all life forms. DNA constructs of two nucleotide strands coiled around each other in a ladder like arrangement with the sidepieces composed of alternating phosphate and deoxyribose units and the rungs composed of the purine and pyrimidine bases adenine, guanine, cytosine, and thymine. Each chromosome consist of one continuous thread-like molecule of DNA coiled tightly around proteins and contains a portion of the 6,400,000,000 basepairs that make up your DNA.

ends when the RNA polymerase reaches a triplet of bases then the DNA molecules re-

After the replication fork has been established the strands of DNA are ready for the next stage. On each strand is a

A stable DNA structure is formed when the two strands are a constant distance apart. This can only occur when a purine (A or G) on one strand is paired with a pyrimidine (T or C) on the other strand. The purine A can only pair up with the pyrimidine T and the purine G can only pair up with the pyrimidine C. Each letter corresponds to a specific nucleotide base. (Wexler 2) A nucleotide is any part of a group of molecules that, when linked together, form the building blocks of DNA or RNA.

3) Copy: DNA Polymerase joins individual nucleotides with their complementary counterparts to form a new strand of DNA. Replication runs in the 5’ to 3’ direction.

Multiple enzymes work together to help your DNA duplicate. Helicase, one of these enzymes used to help replicate DNA, uses energy from the ATP to break apart the hydrogen bonds holding the bases together. This allows for the two parental strands of DNA to unwind and form two replication forks. Primase, also an enzyme used in DNA replication, copies a DNA parental strand by making an RNA strand complementary to it. Primase is only active when another protein is present. There are two DNA polymerases: DNA polymerase III and DNA polymerase I. DNA polymerase III catalyzes the chemical reactions for polymerization of nucleotides. DNA polymerase I is involved in removing RNA after replication. If DNA polymerase makes a mistake during synthesis,

Polymerase I serves to synthesize the new DNA 5’ to 3’ in replication as well as transcription of prokaryotic cells. If the new DNA strand was not synthesized, then no new DNA could be formed, meaning that the cell would most likely die from lack of genetic information. Polymerase I is an initiator enzyme that begins the process by synthesizing a new DNA strand. This is followed by Polymerase II which synthesizes proteins, and then Polymerase III proofreads the entire strand ensuring that no mistakes were made. Without a

There are several enzymes that take part in the DNA replication process. They are Helicase, DNA Polymerase III and Primase. In helicase, this enzyme has several functions in helping make the replication fork so different functions are allowed to occur (Wolfe, 2016). Helicase unravels the double DNA helix to a single stranded template of Adenine, Thymine, Cytosine and Guanine allowing these to be copied (Wolfe, 2016). DNA helicase during single strand separation from the helix also forms the replication fork (Wolfe, 2016). This in turns during the single strand of the nucleotides A, T, C, G still have match together for correct sequence

During this lab the Drosophila cells had to go through certain processes so we could insert the double stranded RNA. The first process is replication. Replication is a semiconservative process in which two strands of DNA are created from one double strand of DNA. The total production is four single strands of DNA, each strand made from one old and one newly made single strand. Replication first starts with a cluster of proteins called a protein complex. When a protein complex binds to a section of DNA with adenine and thymine repeats, called regions of replication, it creates a ‘bubble’ between the DNA. With a small opening in the DNA an enzyme called helicase comes in and makes the bubble larger. In order to keep the DNA from joining back together a protein binds to the open DNA. Primase, another enzyme, lays down little strands of RNA for an enzyme called DNA polymerase III to bind to so it can add new complementary nucleotides to create a new segment of single stranded DNA. DNA Polymerase III can only work

DNA is “the hereditary material in humans and almost all other organisms,” (NIH, 2016). It’s composed of nucleotides which contain a nitrogen base (A, C, G, and T), phosphate group, and a sugar group. The DNA is found within the nucleus where it\s tightly packed into a chromosome. Its structure is a double helix, that’s wrapped around a nucleosome. This structure forms a string of beads that’re coiled about each other. This new structure is now called a thread. Next, the structure is looped, forming a looped chromatin, which will then condense forming a condense chromatin. Furthermore, the genome is formed, which is the complete set of genes or genetic material in an organism. Finally, when the cell breaks down it releases DNA,

Finally, all the nucleotides are joined to form a complete polynucleotide chain using DNA polymerase. The two new DNA molecules form double helices.

Moreover, there are many enzymes that participate in the unwinding of the old strands of DNA molecule such as topoisomerase. This enzyme is responsible for initiation of the unwinding of the old strands of DNA molecule. Once supercoiling has been eliminated by the topoisomerase, helicase accomplishes unwinding of the original double strand. In order to aid with the unwinding process, DNA gyrase catalyzes the formation of negative supercoils. The unwound helix, with each strand being synthesized into a new double helix, is called the replication fork.