Does Manuka Honey Reduce The Turbidity Of Gram Negative Escherichia Coli
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1.1 Research Question:
Does Manuka Honey reduce the turbidity of gram negative Escherichia Coli when mixed into a solution and will it have a larger antimicrobial effect than clear honey? 1.2 Genesis
According to the Centres for Disease Control and Prevention (CDC) a minimum of 2 million people become infected with antibiotic resistant bacteria and an estimated minimum of 23, 000 people die each year as a direct result of those infections . As an increasing number of infectious diseases are becoming resistant to numerous allopathic medicines , for example meticillin-resistant staphylococcus aureusis or MRSA, more research is being conducted into alternative treatments derived from naturally occurring substances. Having heard about the…show more content… A sugar solution and an empty hole in the agar were used as controls.
From the results, it is clear that all of the honeys had a large antimicrobial effect on the culture of E.coli, much more so than the other cultures of bacteria. Their effects on Straphylococcus aureus should also be noted. It is obvious that the clear honey had a negligible antimicrobial effect on all bacterium tested, excepting the E.coli colony. This was to be expected; however, as clear honey lacks the enzymes to create the non-peroxide antimicrobial effects, caused by methylglyoxal, which is present in manuka honey. Therefore as the varying types of honey were most effective on the E.coli culture, it was selected for the main experiment.
Furthermore, during this preliminary testing I experimented with the varying colour filters in the colourimeter, in order to establish which led to the most reliable and measurable results. The results of this experiment was that the blue filter resulted in larger results, meaning that it allowed an appropriate amount of light to reach the honey. Therefore the blue filter will be used in the main experiments, as opposed to the red, green and orange filters.
1.4 Theoretical basis:
Escherichia coli, often shortened to E.coli, is a bacterium in the Enterobacteriaceae family. Although most E.coli strains are