Draw The Fischer Representations Of Each Of Simple Carbohydrate

2. Write a statement to explain the molecular composition of the unknown solution based on the results obtained during testing with the Biuret solution and each sample solution.

Reaction 3- 1. Obtained a clean and dry test tube and placed a small amount ( about the size of a jelly bean) of ammonium carbonate into the test tube.

1. Fill in the table below with the results from the monosaccharide test experiment, and your conclusions based on those results.

When combined Sodium Bicarbonate and Hydrochloric Acid, Carbon Dioxide is produced. The two chemicals do not undergo a change in color but a chemical change when CO2 is produced.

Reducing sugars are monosaccharides that have equilibrium with the straight-chain and cyclic form, which the sugar engages in an oxidation-reduction reaction with Cu2+ (C. Bissonnette, 2011). Through this test, one is able to determine that the unknown solution #128 is a non-reducing sugar because the results show that the test turned out positive, and reducing sugars cause negative tests to occur.

1. List whether each of the following substances was positive or negative for reducing sugar, as indicated by the Benedict's test. (6 points)

7)a) You would do the Benedict’s reagent test for simple sugars and the Lugol’s solution or Iodine test for polysaccharides and starch. To test the lipids you would use the Sudan iV test and Biurets reagent test for protein.

To perform this test, a tube of broth rich with glucose is acquired. In this tube is phenol red, a pH indicator. Initially, the tube appeared pink in color, indicating a normal pH level. Next, a sample of unknown #44 is introduced into this medium using the aseptic technique, and this is allowed to sit for several days. If the organism is able to ferment glucose, the pH in the medium would decrease and cause the phenol red to exhibit a yellow color. In addition to the straw color, gas can also be produced and trapped inside the Durham tube placed in the medium. This production of acid and gas is a direct result of the fermentation of glucose, as seen with unknown

2.List whether each of the following substances was positive or negative for reducing sugar, as indicated by the Benedict's test. (6 points)

To tube 3 a piece of litmus paper was placed into the tube. Then as is tube 2 concentrated HCl was added drop wise until the litmus paper indicated that it is acidic. No CO2 gas will evolve.

The hypothesis stats that as the sucrose concentration is increased, rate of respiration will increase and therefore the CO2 production of yeast cells will rise. Sucrose is a disaccharide composed of the monosaccharaides glucose and fructose. Glucose is a reactant in anaerobic cell respiration. In the absence of oxygen, glucose will react with the yeast producing ethanol and CO2.

It could be concluded that lactose free milk is highly saturated with glucose, giving it a sweet taste and resulting in a constant glucose level at both pH’s. However, rice milk’s sweetness derives from the high concentration of brown rice present; the initial concentration of glucose would be higher than the measurements at pH 4 and pH 1.2 due to the fact that the low pH protonated the carbohydrates present, possibly affecting the structure and lessening the glucose present through denature. The almond milk was not sweetened with glucose at all, resulting in zero glucose as shown in Figure 2; the added pH and enzyme displayed no

To find the effect of temperature on the activity of an enzyme, the experiment deals with the steps as follows. First, 3 mL if pH 7 phosphate buffer was used to fill three different test tubes that were labeled 10, 24, and 50. These three test tubes were set in three different temperature settings. The first test tube was placed in an ice-water bath for ten minutes until it reached a temperature of 2° C or less. The second tube’s temperature setting was at room temperature until a temperature of 21°C was reached. The third tube was placed in a beaker of warm-water until the contents of the beaker reached a temperature setting of 60° C. There were four more test tubes that were included in the procedure. Two of the test tubes contained potato juice were one was put in ice and the other was placed in warm-water. The other two test tubes contained catechol. One test tube was put in ice and the other in warm water. After

Fructose (C6H12O6, purity ≥99%), was from El-Gomhorya Company, Zagazig, Egypt. Nigella Sativa oil was obtained from Pharco Company, Egypt. Chromium picolinate was purchased from.

Sugars exist in solution as an equilibrium mixture of open chain and closed ring(orcyclic), strutures. sugars that can be oxidised by mild oxidising agents are called reducing sugars because oxidising agent is reduced in the reaaction. A non reducing sugar is not oxidised by oxidising agent . All common monosaccrides are reducing sugars. there are several test forr sugars which are based on composition or specific group. Hence sucrose is not a reducing agent due to bonding of oxygen with carbon to form glycosidic bonds between monosacrrides. Therefore they are not free to let go so cant be reduced , some of test includes seliwanoff 's test which distinguish between aldose and ketose sugars.Other test for sugars is benedicts test which commonly used to detect presence of