Drosophila Autosomal and Sex-Linked Cross The idea of the project was to experiment breeding Drosophila Melanogaster (fruit fly) to figure out if certain genes of that species were sex linked or not (autosomal). A mono-hybrid cross and di-hybrid cross was performed. For the mono-hybrid cross, white eyed female and red eyed male were placed in one vial for them to reproduce. For the di-hybrid cross, red eyed and normal winged flies and sepia eyed and vestigial winged flies were placed in their vial to reproduce. In the mono-hybrid cross the results expected were within a 1:1:1:1 ratio. Expected results similar to the expected desired null hypothesis proposed with what the F1 parental generation breeds. The potential results would have had…show more content… His second theory was the law of independent assortment which occurs in metaphase I. This theory was construed based on the fact that a new phenotypic ratio occurred in the F2 generation with a di-hybrid cross (9:3:3:1). Here, alleles (genes) are expected to segregate independently from each other (Cain, Urry, & Reece, 2010). Thomas Hunt Morgan continued Mendel’s work and in 1911, decided to experiment on Drosophila melanogaster, also known as fruit fly. His study on them was due to the fact that they had only 4 chromosomes which 3 were autosomal and 1 was a sex chromosome. His study proved that eye color was sex linked (Cain, Urry, & Reece, 2010). Other traits were not linked. Any mutation can change the result of a mutated gene which would give variations. Such is the case in the eyes of the fruit flies. The purpose of this experiment is to see if Mendel and Morgan both had valid points. Examining the fruit flies all the way to the F2 generation would give way to prove or disprove Mendel’s expected results and Morgan’s visual results.
Method and Materials
A three week experiment was proposed. On week 1, the preparation of vials was the beginning of the experiment. An equal amount of food and water was measured and then mixed. This mixture was to be the food for the larvae. The mixture was placed in the vile with a height of 2-3 cm. The mixture had to be placed inside and made sure by pressure that no air pockets were