Drosophila Fruit Fly

This may have been the cause of the low numbers of white lozenge in the F2 generation of flies. However, the cause of white eyes is a defective red pigment gene and should not affect the vision of the flies, whereas the lozenge gene should have a greater affect due to it causing the malformation of the fly's eyes. Therefore the lozenge flies should have also been in lower than expected numbers, but it was found that they were actually in higher than expected numbers making the validity of this argument questionable.

Drosophila melanogaster is a small, common fly found near unripe and rotted fruit. It has been in use for over a century to study genetics. Thomas Hunt Morgan was the best biologist studying Drosophila early in the 1900’s. Morgan was the first to discover sex-linkage and genetic recombination, which placed the small fly in the forefront of genetic research. Scientists have used Drosophila for many reasons. For one they are very easy to maintain, breed, anesthetize, and kill with little equipment. They are also very small and it is easy to distinguish males vs females and sexually mature flies and virgins. At lastly, the flies have a very short two week life span. On days 2-7 of their life

It was decided that there would be 80 vestigial flies and 20 wild type flies to total to an initial population of 100 drosophila. Next, the flies were anesthetized flies using Fly Nap. The flies were counted out to reach desired ratio, sexing the flies making sure there are equal amounts of males and females to be sure there is ample individuals to allow successful mating. The fly’s food was prepared by taking a frozen rotten banana, cutting it in half, mashing up the banana meat, and mixing yeast into it. The

Introduction: The intention of this lab was to gain a better understanding of Mendelian genetics and inheritance patterns of the drosophila fruit fly. This was tasked through inspecting phenotypes present in the dihybrid crosses performed on the flies. An experimental virtual fly lab assignment was also used to analyze the inheritance patterns. Specifically, the purpose of our drosophila crosses is to establish which phenotypes are dominant/recessive, if the traits are inherited through autosome or sex chromosomes and whether independent assortment or linkage is responsible for the expressed traits.

The expected number of wild type flies in the F2 generation is 734.25 and the expected number of shaven bristle flies in the F2 generation is 244.75. This, again, exhibits a 3:1 ratio of normal phenotype to affected phenotype.

Describe the sex and phenotype of the mutant fly. Describe the phenotype as it compares to the wild type.

Mature flies have no functioning mouth-parts and are not associated with transmission of diseases or considered pests

This lab had 2 exercises. Exercise 9.1 involved observing pictures of 60 F2 offspring and recording the phenotypes for 6 different traits. Exercise 9.2 required us to perform the “chi-square test” to determine whether the data we collected matches the standard Mendelian ratio.

Table 2 shows the phenotypes of the F1 flies produced by crossing P1 wild-type males and P1 no-winged females. The results of that cross was that there was fifty nine wild-type females and forty one males. Therefore there was a total of one hundred wild-type flies produced from crossing P1 true breeding wild-type males and P1 true breeding virgin

11. The progeny of a Drosophila female (heterozygous at three loci: y, ct, and w) crossed to a wild type male are listed below:

METHODS: In this experiment, the instructor provided us with 30 ebony individuals and 20 wild type individuals. In order to get an exact amount of each type, we anesthetized the flies and counted them off by gently using a fine point paint brush. Then all 50 Drosophila were put into a population cage which had a lid that had six holes for the centrifuge tubes. Two food tubes and four clean, empty tubes were added on the first day. Each food tube consisted of half a cup full of food mixed with 6-7 milliliters of water. This was the fly medium. The food should turn blue once the water is added. Each tube was labeled with a number and with the date. Every two to three days we added one more food tube until all 6 tubes contained the fly medium. After all 6 tubes were filled, the following days after we exchanged the first food tube with a new food tube. At the end of the experiment, we fed the flies with a total of 8 food tubes. Then the flies were anesthetized, again. At the end of this four week lab, the number of living ebony and wild

we said goodbye and placed them in the fly morgue. We allowed the F2 larval

In most kitchens the small flies that are found are Drosophila Melanogaster also called fruit fly. They are often brought in by ripened tomatoes, grapes and other perishable items from the garden. Drosophila melanogaster is a little two winged insect about 3mm long two winged insect that belongs to the Diptera, the order of the flies. The drosophila egg is about half a millimeter long. Fertilization takes about one day the embryo to develop and hatch into a worm-like larva. The larva eats and grows continuously, after two days as a third in star larva; it moults one more time to form an immobile pupa. Over the next four days, the body is completely remodeled to give the adult winged form, which then hatches from the pupal

The vial was then labeled accordingly with the type of cross (Male Vg, Female W) and the date. The date is important as the Drosophila complete a life cycle within approximately 2 weeks from the mating day. This vial became known as the parent generation or (P).

The spotted wing Drosophila suzukii (Diptera: Drosophilidae) is an attacking pest of berries crops. Unlike most other Drosophila, this insect can oviposit into ripe and ripening berries, so that they make them unmarketable. D. suzukii is spreading quickly throughout the continental US and they give a serious damage in horticultural areas and the fruit industry. Spotted wing drosophila (SWD) puts its eggs in both commercial fruit and wild fruits, so it is of great concern to fruit and vegetable growers. It became a pest of economic significance, but it is important point that requires early detection, monitoring and moderation to control it. Last researches show that D. suzukii infests a number of wild plant species and also those may be harvested commercially like cranberry. Gray dogwood (Cornus racemose Lam.), Tatarian honeysuckle (Lonicera tatarica Linneaus), Mulberry (Morus alba L.), Nanking cherry (Prunus tomentosa L.), Common chokecherry (Prunus virginiana L.), Common buckthorn (Rhamnus cathartica R. Davurica), Elderberry (Sambucus Canadensis L.), White snowberry (Symphoricarpos albus L.), Yew (Taxus