Drosophila Lab Paper

It is possible that the temperature at which the flies were kept dropped significantly below 20 degrees Celsius; this could have caused the death of some of the files or slowed down their growth and reproduction rate. This would result in there being fewer flies. This problem could be overcome in further experiments by using a larger heating device with a more responsive thermostat to keep the Fly house within the recommended temperature range for D. melanogaster.

The parents are both homozygous. The homozygous dominant would represent the wild type. And the homozygous recessive would represent the other fly parent of a different strain. The F1 generation would consist of 100% Wild Type but they would all be heterozygous in carrying the recessive gene.

With the presence of natural selection (fly paper hanging from top of cage) posing a larger threat to the wild flies b/c they can fly better, this will allow vestigial flies to prosper better.

Introduction: The intention of this lab was to gain a better understanding of Mendelian genetics and inheritance patterns of the drosophila fruit fly. This was tasked through inspecting phenotypes present in the dihybrid crosses performed on the flies. An experimental virtual fly lab assignment was also used to analyze the inheritance patterns. Specifically, the purpose of our drosophila crosses is to establish which phenotypes are dominant/recessive, if the traits are inherited through autosome or sex chromosomes and whether independent assortment or linkage is responsible for the expressed traits.

The expected number of wild type flies in the F2 generation is 734.25 and the expected number of shaven bristle flies in the F2 generation is 244.75. This, again, exhibits a 3:1 ratio of normal phenotype to affected phenotype.

It would be expected that the mutant F1 flies would be heterozygous for the allele responsible for the grounded trait. If two F1 flies were mated, the percentage of flies that would be expected to be wildtype in the F2 generation would be 25% mutants given that the mutant allele (ap) is predicted to be recessive and, leaving 75% to be wildtype (ap+).

Table 2 shows the phenotypes of the F1 flies produced by crossing P1 wild-type males and P1 no-winged females. The results of that cross was that there was fifty nine wild-type females and forty one males. Therefore there was a total of one hundred wild-type flies produced from crossing P1 true breeding wild-type males and P1 true breeding virgin

11. The progeny of a Drosophila female (heterozygous at three loci: y, ct, and w) crossed to a wild type male are listed below:

Environmental conditions are sometimes appropriate for the evolution of numerous species from a common ancestor. Evolutionary, or adaptive, radiations happen when a large number of species descend from one lineage. This phenomenon usually occurs when a population of one species colonizes an area with many available ecological niches and when the rate of speciation is greater than the rate of extinction in a given area. Adaptive radiations have historically taken place after mass extinctions and on newly formed island archipelagos, because these incidences create conditions where new species can fill open ecological niches. Unlike the mainland, ecological niches are available on new island systems. These island species adapt to new environments

A. The flies in the rural boxes were devoured by its predator (Spalangia), these small wasps, lay eggs into immature fly pupae after hatching, the wasp larvae feeds on the developing fly, killing it. This study suggests that stable flies are vulnerable out in the rural pastures rather than inside the feed lots where

Fruit flies with normal wings have a higher population and greatly outnumber the population of fruit flies with dichaete or vestigial wings.

To start, a fly anesthetic was used named “FlyNap” to anesthetize the drosophila melanogaster. A fur wire was then dipped into the FlyNap and then placed into the vial with the vial on its side being careful not to uncork the foam plug. After about a minute the drosophila melanogaster become unconscious. The unconscious drosophila melanogaster are then swept onto a plate with a small paintbrush. Once they are on a plate they are then to be scored with a compound microscope. For the experiment five females and five males were required to be placed in vial with at the bottom of this vial were some parts dry fly food, mixed water, and granules of yeast. While keeping the vial sideways sliding a small portion of plastic netting and then

Some larva containing vials had hatched into flies. Counting of the flies began at this point. As flies started to grow, at different rates for each vial, with in the first seven days after all larva had hatched the flies were counted. The procedure was done according to theDrosophila manual (45-2620)

Drosophila melanogaster was chosen for the experiment as it is the archetype for population genetics. Drosophila is a model organism for population genetics just as the house mouse has become a model organism (Powell, 1997). The benefits of using Drosophila in this experiment is that it has a short life cycle along with a fast

Drosophila melanogaster stocks used for the behavior assays (2-choice assay and tracking assay) and molecular analysis (qRT-PCR and immunohistochemistry), include the wild type Canton-S (CS) line, the UAS-GABA (B)-RX-RNAi (Root et al., 2008) (where X represents receptor subtype 1, 2, or 3), and Or X-Gal4 lines (Or 47a Gal4 and Or 42b Gal4), GH-146 Gal4, Orco-Gal4, 10x; UAS-CD8; GFP were purchased from the Bloomington stock center (http://flystocks.bio.indiana.edu). Virgin female flies from UAS-GABA (B) R1-RNAi line were crossed to males from Or 47a Gal4, also UAS-GABA (B) R1-RNAi females were crossed to males of Or 42b Gal4. Similar crosses were made using UAS-GABA (B) R2-RNAi and UAS-GABA (B) R3-RNAi lines and Or 47a and