Methods and Materials: Primer Design and Cloning: To explore regions of the Drosophila genome that weren’t categorized by Stark lab fragments, we used a molecular approach to design novel candidate enhancers from the ChIP sequence peaks. This approach also allowed us to create narrower fragments (approximately 1000 bp), focusing on enhancer activity and reducing the chance of background activity. Fragments containing in vivo Bcd and Otd binding signals were selected by searching between 500 bp
Drosophila Project Reflection Paper Being an effective group member in an upper level class takes a lot of communication and effort from everyone involved if the group wishes to succeed. All parties involved need to be on the same page and able to coordinate with each other effectively. When it comes to lab work like we performed in the Drosophila project, there were multiple levels of communication involved. First off, just working together effectively in lab took patience. Making sure everyone
Introduction In most kitchens the small flies that are found are Drosophila Melanogaster also called fruit fly. They are often brought in by ripened tomatoes, grapes and other perishable items from the garden. Drosophila melanogaster is a little two winged insect about 3mm long two winged insect that belongs to the Diptera, the order of the flies. The drosophila egg is about half a millimeter long. Fertilization takes about one day the embryo to develop and hatch into a worm-like larva. The larva
Natural and Sexual Selection of Vestigial and Wild Type Drosophila melanogaster Abstract In this experiment, vestigial flies as well as wild type flies were used to create some diversity as well as test one of our hypotheses. Our hypotheses are as follows; 1) The wild type flies will have a greater relative fitness compared to the vestigial flies based on only sexual selection. The wild type has a relative fitness of 1 since it has fully functional wings. Vestigial Winged flies are not as sexually
Abstract Drosophila melanogaster was used for this study for their fast reproduction cycles, fast regenerations, large amounts of offspring for each generation and their capability of living in a small limited space. The dominant or recessive genotype could be determining by the used of Mendelian genetic ratios for wild-type to mutant’s genes. The mutation that this study focuses on is the defects of the phenotypes in the common fruit fly, example; wing shape, wing sizes, body color and what
post-transcriptional RNA editing and its function. RNA editing has been demonstrated to be necessary for proper development and function, but its role is not well understood. The first paper, “Cis Regulatory Effects on A-to-I RNA Editing in Related Drosophila Species,” compares Adenosine-to-inosine (A-to-I) RNA Editing between two Drosophila species: D. melanogaster and D. sechellia. The primary interest of this study is the regulation of RNA editing and whether this regulation is determined predominantly by cis
Kasey Sammis Tracing the Genetics of a Fruit Fly Intro: In this laboratory experiment we cross bred fruit flies (Drosophila melanogaster) in order to identify which traits were passed onto their offspring. Since the 20th century the common fruit fly has been a useful and one of the best organisms for the study of genetics. Thomas Hunt Morgan was responsible for the work that demonstrated the huge advantages of fruit flies and led to them becoming a common model for scientific research and testing
665 7.816 11.345 Title: Mendelian Genetics in Drosophila Melanogaster Amber Coleman and Shyla Coleman 0001 MC Genetics Lab Spring 2015 April 30th, 2015 Objectives: • Attempt to introduce the characteristics of the normal “wild type” to the different mutant phenotypes in Drosophila. • To determine the ratio of different crosses learned, such as, monohybrid ross, dihybrid cross and sex linkage cross of Drosophila melanogaster. • To introduce the many uses of the Chi square statisitic
Scientific Literature Worksheet (Due Lab 2) Upload completed Worksheet to SafeAssign and bring a hard copy to lab for grading Name ________Ivy Wu__________ TA ____Lauren Prentis_____ Section _22__ What is the topic number of your assigned article and the title of the paper? #12 Title: “Genetic Dissection of Mechanosensory Transduction: Mechanoreception-Defective Mutations of Drosophila” Outline of the Main Points to be included in your Poster Presentation of your article WHY: Briefly describe
Fruit Flies: A Genetic Analysis of Inheritance in Drosophila melanogaster Introduction Mendelian Genetics Gregor Mendel, the father of genetics, discovered principles of inheritance through breeding peas of different color and texture. He crossed several types of peas to investigate dominance relationships, variability, and genetic probability. Through his experiments, he laid down the foundation of inheritance that geneticists use to this day (Griffiths, 2015). From these crosses, Mendel pioneered