Drosophila Melanogaster Dumpy

Butterflies have many genes which are expressed into ways that are either dominant, or recessive. For example to have blue eyes the dominant allele would be (B) and the recessive allele would be (b).

The parents are both homozygous. The homozygous dominant would represent the wild type. And the homozygous recessive would represent the other fly parent of a different strain. The F1 generation would consist of 100% Wild Type but they would all be heterozygous in carrying the recessive gene.

The goal of this study was to induce a deletion in the DMAP1 gene on chromosome two in Drosophila melanogaster through P-element mobilization. The DMAP1 gene may be an essential gene, however not much is known about it. We attempted to uncover the function of DMAP1 by creating a series of genetic crosses and selecting for brown-eyed non-stubble male flies that may have the deletion. To test whether these flies had the deletion, we produced PCR products and ran them on an agarose gel, which resulted as inconclusive. We created a balanced stock of flies homozygous for the deletion to see if the

Introduction: The intention of this lab was to gain a better understanding of Mendelian genetics and inheritance patterns of the drosophila fruit fly. This was tasked through inspecting phenotypes present in the dihybrid crosses performed on the flies. An experimental virtual fly lab assignment was also used to analyze the inheritance patterns. Specifically, the purpose of our drosophila crosses is to establish which phenotypes are dominant/recessive, if the traits are inherited through autosome or sex chromosomes and whether independent assortment or linkage is responsible for the expressed traits.

Drosophila Melanogaster, commonly known as fruit flies, are highly important model organisms in pertaining to biological research. The logic behind their recurrent use is due to their: easy culture in the laboratory, brief generation time, and ability to produce large numbers of offspring. In this report, we created isolated virgin D. Melanogaster from the original three populations we were given and then created crosses between them. Upon observation, we noticed an unusual mutant that arose from two of the three created crosses. We suspected that this genetic mutation had previously been discovered and named.

The motivation of this lab report is to use Mendel’s Laws of Inheritance to analyze and predict the genotypes and phenotypes of an offspring generation (F2) after knowing the genotypes and phenotypes of the parent generation (F1). The hypothesis for this experiment is that the mode of inheritance for the shaven bristle allele in flies is autosomal recessive in both male and female flies.

Jack J, DeLotto Y. Effect of Wing Scalloping Mutations on Cut Expression and Sense Organ Differentiation in the Drosophila Wing Margin. Genetics. 1992;131(2):353-363.

Roi1 is also known as rough eye and it is a dominant mutation which causes abnormal patterns and genomic inversions in the D.melanogaster eye (Chanut et al. 2002). The recombination map location of the Roi1 is 2-54.7. The gl3 is an allele and the Gl1 is a protein for the gl gene, also known as glass, both are located at 3-63.1. The gl gene is known to reduce the size of the adult D. melanogaster eye. Even though the gl3 is a weak allele for the glass gene it produces a really pigmented eye ( Ma et al. 1996). The rh1 gene is known as rhodopsin; the recombination map location is at 3.66.4. Rh1 causes degeneration of the D. melanogaster retina (Kristaponyete et al. 2012). Rho1 also known as the rhomboid gene, is closely related to the roughed (ru); which happens to be a recessive eye mutation (Wasserman

Gain of function (ectopic expression in the developing haltere epithelium) of each of these genes changes the haltere of the fly into a second set of wings: double-wings phenotype (GOF).

The most extensive metamorphic changes of insects internal organs occur with development of the thoracic musculature required for flight, the arrangement of nervous system and the development of gonads [2].

we said goodbye and placed them in the fly morgue. We allowed the F2 larval

This experiment looks at the relationship between genes, generations of a population and if genes are carried from one generation to another. By studying Drosophila melanogaster, starting with a parent group we crossed a variety of flies and observe the characteristics of the F1 generation. We then concluded that sex-linked genes and autosomal genes could indeed be traced through from the parent generation to the F1 generation.

Melanogaster. Although leakiness was proven within this system, it has practical uses for the temporal control of gene expression. If we were to utilize this system in our research with Dr. Beckingham, it would be advisable to test controls for the system in the type of driver we want to use and the genes we want to knockdown in order to account for the system’s leakiness. An important application of the GeneSwitch system is the possibility of it being a great mechanism for studying the effects of overexpression of multiple genes while other genes are being knocked down at the same time. In Beckingham's lab, with further research in Drosophila, we may come across points at which we would like to control the time at which certain genes are expressed or depleted rather than the area at which they are. We also may want to have the means to knock down or express multiple genes, and the GeneSwitch Gal4 system would be a practical way to accomplish these goals in further

Hypothesis: The hypothesis is that Normal wings (NN) are the dominant trait and Butterfly wings (bb) are the recessive trait. The original parents are purebred (homozygous dominant (NN) and homozygous recessive (bb). The F1 generation is predicted to displays 100% Normal wings population of heterozygous (Nb). The F-2 generation is predicted to display a 3:1 Mendelian ratio of Normal wings to Butterfly wings.

The purpose of this lab was to develop the understanding of the inheritance patterns using fruit flies, Drosophila Melanogaster. We chose Drosophila Melanogaster because of the fact that they are small and are an easy use in the laboratory. As well, they have qualifying characteristics to be able to use. They are very easy to distinguish the differences between female and males, have a short life cycle, inexpensive, and understandable traits to be a perfect example. Also, fruit flies produce a large amount of offspring, which helps us out when finding out pattern of inheritance (Morris and Cahoon, p. 5). In