Drosophila Melanogaster Lab Report

Introduction: The intention of this lab was to gain a better understanding of Mendelian genetics and inheritance patterns of the drosophila fruit fly. This was tasked through inspecting phenotypes present in the dihybrid crosses performed on the flies. An experimental virtual fly lab assignment was also used to analyze the inheritance patterns. Specifically, the purpose of our drosophila crosses is to establish which phenotypes are dominant/recessive, if the traits are inherited through autosome or sex chromosomes and whether independent assortment or linkage is responsible for the expressed traits.

This determination was mainly so the group could easily spot the sex and traits of the flies for later steps. This was done to all four tubes of the P generation, all of the adult females were placed disposed of roughly 30 males from the wild type were kept in a separate jar. The adult females were not wanted because the females could have already mated with the males and the group needed the control of the transfer of genetics for the test crosses. The new virgins were born and collected approximately eight hour after the removal of the adults and placed into separate jars labeled by the genotype of their parents. After the process was done and 5-6 virgin females were collected the process could continue in order to cross still the P generation.

Introduction For centuries, researchers have used Drosophila melanogaster, the common fruit fly, to study genetics. The benefits of using the fruit fly includes: its relatively short generation time, its large amount of available offspring for data, it is easy to store and handle in the laboratory and it is easily and cheaply obtained. Cross-breeding of four types of fruit flies were used in this experiment including: wild type males with normal wings vs. vestigial wing females, wild type males with red eyes vs. white eyed females, wild type male with red eyes vs. sepia eyed females, and wild type males vs. wild type females. In basic mendelian genetics, the terms dominant, recessive and sex-linked are used to describe the different types

Describe the sex and phenotype of the mutant fly. Describe the phenotype as it compares to the wild type.

we said goodbye and placed them in the fly morgue. We allowed the F2 larval

Studies on the genetics of other animals, such as fruit flies, can reveal more about the genes of other animals, including humans. After crossing the flies, a hypothesis can be made based on the data obtained on whether the traits are autosomal, sex-linked, recessive, or dominant. Methods Prior to this experiment, the vials used to hold the Drosophila melanogaster were made.

11. The progeny of a Drosophila female (heterozygous at three loci: y, ct, and w) crossed to a wild type male are listed below:

This experiment looks at the relationship between genes, generations of a population and if genes are carried from one generation to another. By studying Drosophila melanogaster, starting with a parent group we crossed a variety of flies and observe the characteristics of the F1 generation. We then concluded that sex-linked genes and autosomal genes could indeed be traced through from the parent generation to the F1 generation.

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Aim: to establish whether characteristics produced from the offspring of a drosophila cross are recessive or dominant traits.

The parents are both homozygous. The homozygous dominant would represent the wild type. And the homozygous recessive would represent the other fly parent of a different strain. The F1 generation would consist of 100% Wild Type but they would all be heterozygous in carrying the recessive gene.

The pairs of alternative traits examined segregated among the progeny of a particular cross, some individuals exhibiting one traits, some the other

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For our first generation (F1) of flies we chose to cross apterous (+) females and white-eye (w) males. We predicted that the mutation would be sex linked recessive. So if the female was the sex with the mutation then all females would be wild type heterozygous. Heterozygous is a term used when the two genes for a trait are opposite. The males would all be white eye since they only have one X chromosome. If the males were the sex that had the mutation then all the flies would be wild type but the females would be heterozygous.