Introduction Have you ever wondered how specific traits are passed down from generation to generation? Or if you already know the answer to that question, then how can you determine which traits are dominant and recessive. Finding both answers can be obtained by studying genetics. Reading about these topics only gives you a grasp on how traits work. In a laboratory setting, the answers can be found in an experiment using an unlikely specimen, known as the common fruit fly and its scientific name, Drosophila Melanogaster. What makes D. Melanogaster perfect for the experiment is the two week lifespan and their ability to reproduce in high numbers. While using D. Melanogaster in an experiment like this, reproduction can be manipulated through …show more content…
Melanogaster species was performed to track gene linkage and inheritance patterns to help us better understand these concepts. After the experiment is finished we should be able to determine the ratios and phenotypes of each cross and can determine which traits are dominant and recessive. The biggest part of this experiment is to determine whether the traits are sex-linked or inherited independently. We’ll say the genes are sex linked because if we used the female wild type virgins we should be able to see if certain sexes in the progeny are more prone to showing these traits. In the first cross, a wild type female is crossed with a male that has sepia eyes and an ebony body. In the second, a wild type female is crossed with a male that has white eyes, a yellow body, and miniature wings. In the third and final cross a wild type female is crossed with a male showing the traits of sepia eyes and vestigial wings. Seeing how these crosses have been conducted, a common question is why the wild types in the cross are females. Females are used due to the flies being able to reproduce after they hatch from their pupae stages within eight hours. If we use virgin females then we will be able to more accurately obtain results (www.mun.ca/biology/dinnes/B2250/DrosophilaGenetics.PDF). Results will be derived from a chi-square analysis. We’ll conduct the experiment with the assumption that
The motivation of this lab report is to use Mendel’s Laws of Inheritance to analyze and predict the genotypes and phenotypes of an offspring generation (F2) after knowing the genotypes and phenotypes of the parent generation (F1). The hypothesis for this experiment is that the mode of inheritance for the shaven bristle allele in flies is autosomal recessive in both male and female flies.
4. Clear wing, Black eye, and Hairless (c, b, and h) are linked, recessive traits carried on
Table 1 shows the phenotypes of the F1 flies produced by crossing P1 wild-type females and P1 no-winged mutant males. The results of that cross was that there were forty seven wild-type females and fifty three wild-type males. Therefore there was a total of one hundred wild-type flies that were produced. The observed phenotypic ratio of wild-type flies and no-winged mutant flies was 1:0 (wild-type: no-winged). The predicted phenotypic ratio if the no-winged mutation is autosomal recessive would be 1:0 (winged: no-winged).
When examining the D. Melanogaster mutants in the lab, our group immediately noticed an apparent difference from the wild-type flies. None of the mutants were able to fly. This led us to believe that we were dealing with a wing mutation. Upon further examination, we concluded that it was the overall wing shape that prevented the mutants from flying. The wing shapes among the mutants varied in both size and shape. Some were long, while others were short. The mutant wings could be distinguished into two general classes. One division of the mutant wing was short and stubby, almost a fourth the size of the wild-type wing. The rest of the mutants ranged in wing size and length, however many mutant wings were the same length as the wild-type wings. Although the
Heterozygotes, which have the wild type phenotype, have normal sight which gives them the advantage of finding a mate and have a better success with attracting a mate with their courtship song (Kyriacou et al, 1978). The male heterozygous Drosophila had a better advantage at mating than the homozygotes, which were the ebony, and therefore we predict there will be more wild type by the end of the experiment.
The main purpose of this lab was to utilize the infamous Hardy-Weinberg equilibrium equation to predict the evolutionary modifications a certain species (Drosophila melanogaster) displayed throughout different generations. For this experiment to be carried out, Drosophila melanogaster, also known as fruit flies, were used to visually represent evolutionary conceptions such as Hardy-Weinberg equilibrium equation. At the beginning of the experiment, the parent generation was observed first. Throughout the course of seven weeks, the vial was analyzed for certain changes between the two populations of Drosophila melanogaster; wild type and ebony. Although the genotypes could not be figured out, the flies were evaluated and observed based on
The purpose of this lab is to determine the genotypes of the flies and the random chance involved in inheritance along with the effect of genetic recombination on inheritance. Drosophila melanogaster, the common fruit fly, is commonly used for genetic studies because of its minimal inherited traits, quick life cycle, and fast reproductive rate. Punnett squares were used to predict the allele composition of the offspring resulting from P and F1 crosses. The two genes observed in the lab were eye color and body color. The parental cross was between a female with a yellow body and white eyes crossed with a male with a grey body and red eyes.
Fruit Fly experiment was conducted by using different techniques. One of the main things was to examine the fruit flies and identify the difference between females and males, identify their mutation if they were wild type, white eye, vestigial or white and vestigial combined together. These Fruit flies were kept in the incubator at 25°C for about 6 days. The main goal for this experiment was to observe the principles of Mendelian genetics.
This experiment looks at the relationship between genes, generations of a population and if genes are carried from one generation to another. By studying Drosophila melanogaster, starting with a parent group we crossed a variety of flies and observe the characteristics of the F1 generation. We then concluded that sex-linked genes and autosomal genes could indeed be traced through from the parent generation to the F1 generation.
Genetic Crosses In Drosophila melanogaster Edison Nurcellari, Norma Sutton, Lisa Brown Abstract: The purpose of this experiment is to see how characteristics such as ( phenotypes) are transmitted from parents to offsprings by using true breeding stocks. While doing more than two test crosses from the parent the alleles are re-shuffle so the offsprings get different variations sets of allele then the parents. This process is known as genetic recombination which occurs during meiosis were chromosomes break and exchange genetic material. Another cause of offspring to receive certain allele combination is also due to natural selection for survival and reproduction.
Scientists all across the world have used model organisms to study genetics. These organisms are selected based off of easily noted physical characteristics, their small sizes and relatively short generation time, and also being readily available to use and tractable. That is why Drosophila melanogaster is a great model organisms because it fits all these characteristics. I propose further research on sex-linked eye color mutations in D. melanogaster because studying sex-linked traits will
The study of population genetics is simply how the genes or alleles within a population may vary or change. The purpose of population genetics is being able to predict the genes that are expressed in future generations and understanding why that is. An allele is an alternate form of a gene, so blonde hair and black hair are both alleles but hair color is a gene. In this particular experiment the alleles being focused on are the body colors of the Drosophila melanogaster, which is the common fruit fly. The two different body color alleles are the wild type, tan body color, and the ebony type, black body color.
For our first generation (F1) of flies we chose to cross apterous (+) females and white-eye (w) males. We predicted that the mutation would be sex linked recessive. So if the female was the sex with the mutation then all females would be wild type heterozygous. Heterozygous is a term used when the two genes for a trait are opposite. The males would all be white eye since they only have one X chromosome. If the males were the sex that had the mutation then all the flies would be wild type but the females would be heterozygous.
Drosophila melanogaster was used for this study for their fast reproduction cycles, fast regenerations, large amounts of offspring for each generation and their capability of living in a small limited space. The dominant or recessive genotype could be determining by the used of Mendelian genetic ratios for wild-type to mutant’s genes. The mutation that this study focuses on is the defects of the phenotypes in the common fruit fly, example; wing shape, wing sizes, body color and what the main focus of this experiment is dark eye pigment of the flies. These mutations were followed for three generations, collectable data for wild-type and mutants was obtained for each of the Drosophila melanogaster generations. The flies were
The father of modern genetics, Gregor Mendel is accredited for discovering the basic principles of genetics, he is most known for his experiment with peas which later lead to the study of heredity Mendel most important conclusions where what we know today as principles of Mendelian inheritance which consisted of two principles the law of random segregation and the law of independent assortment (Gregor Mendel Biography.com, 2017). In this experiment, we utilized Drosophila melanogaster to study mutant phenotypes, observed basic patterns of Mendelian inheritance, and develop a hypothesis based on the cross results. The objectives of this experiment are to analyze drosophila mutants by observing the phenotypes, also to observe the expected patterns of Mendelian inheritance, develop a hypothesis on the mode of inheritance and phenotypic ratios, and finally analyze the results using a Chi-Square Analysis (Department of biology 2017). We predicted in our hypothesis that in the monohybrid cross of Drosophila melanogaster, the phenotypic ratio would be of 3:1 for the F2 generation