E Coli : A Public Health Concern

Esherichia coli also known as E. coli is a bacterium that lives in your gut. (1). it was founded by Theodore von Esherich in 1888. There are many people that can get the bacteria. Also there are thousands of strands of E.coli. Six E. coli O157 outbreaks were identified during 2007. Four of the outbreaks involved foodborne transmission. (Eshericha Coli). Six Minnesota cases and one Wisconsin case with the same or closely-related PFGE subtype of E. coli O157:H7, and an additional Minnesota case of Shiga-toxin producing E. coli that was not culture-confirmed, attended the Minnesota State Fair in August. All but one of the cases showed cattle or visited the cattle

Escherichia coli are rod shaped, gram negative bacteria often found in the gut of humans and other warm-blooded mammals (Jacques & Ngo, 2004). It is transmitted primarily through faecal contaminated food and water. Most strains of E. coli are not harmful to humans, however some strains are pathogenic and can cause symptoms including diarrhoea (Unknown, 2012).

On August 19th, 2015 this experiment was performed, by 6 separate lab groups.The experiment began by measuring 1 Ml of E. coli into a pipette and pump, then placing the bacteria into a culture medium. The E. coli and medium were then swirled together for a period of 15 minutes, until completely mixed. This mixture was then poured into a petri dish and allowed to solidify for 45 minutes. After the 45 minute solidification time, 5 small paper disks were inserted into the dish. 4 of the disk contained treatments of antibiotic and 1 was left untreated. The

Escherichia coli (E. coli) is a bacteria usually found in warm blooded organisms such as human and animal intestines. Although there are large groups of E.coli, most strains are harmless. Other strains of the bacteria may cause diarrhea, respiratory illnesses and food poisoning. Some strains such as O157:H7 can cause kidney failure or severe anemia. Exposure to E.coli can be in the form of food, water, undercooked meat and even raw vegetables. Symptoms may include: nausea and vomiting, severe stomach cramps and stomach tenderness, diarrhea containing blood.

coli bacteria is a type of bacteria that can live in the intestines of animals and humans. However, there are some types of the E. coli bacteria, particularly E. coli 0157:H7, that can cause intestinal infections. Symptoms of this type of infection include dehydration, abdominal cramps, fever and bloody diarrhoea. These symptoms can endure 2-8 days after exposure to the bacteria. Since these symptoms generally resolve after a few days to a week, most cases of E. coli 0157:H7 infection can be treated at home.

Escherichia coli (E.coli) has been a public health issue since the 1980s and is a major concern in food contamination in products like beef, dairy, and fresh produce. E.coli 0157:H7 itself is not invasive but it produces a Shiga toxin which attacks the host small blood vessels in the kidney. This makes the kidneys work harder which can destroy red blood cells and lead to hemolytic anemia (lowered number of red blood cells produced). The incubation period for E. coli ranges from three to eight days. The infection often causes severe bloody diarrhea and abdominal cramps, but can also cause non-bloody diarrhea or no symptoms. E.coli may greatly affects children and the elderly.

Escherichia Coli, or E. coli, resides in the large intestine where it assists digestion and make up essential molecules like Vitamin K. For the most part, E. Coli poses no threat although there are few disease causing strains that can cause mild to severe food poisoning by finding its way into food supply (Timmons, Trzepacz, Duboinis-Gray). Enteropathogenic Escherichia coli, or EPEC, is a strain of diseasing causing E. Coli that is the leading cause of death in children with diarrhea in many parts of the world. EPEC colonizes in the intestine allowing the host to be invaded which can spread from person to person (Pearson, Wong fok Lung, Hartland, Giogha). Another strain of disease causing E. Coli also causes diarrhea in children is the DEC

Escherichia coli, oe e.coli, is a safe bacteria found in the large intestine, but also a dangerous bacteria outside of the body. While inside the body the bacteria produces vitamin K which cannot be obtained from food. Therefore e.coli is an essential bacteria in the human body. This bacteria is also a facultative anaerobe meaning it can live with and without oxygen. This means that it can thrive inside of the body, but it can also thrive outside of the body. When e.coli is outside of the body it is very dangerous. E.coli can be found in uncooked meats, unpeeled vegetables, unwashed fruits, and everything contaminated with feces. Dr. Harrlet Burge (2011) stated that e.coli can survive in water for up to 100 days and can remain in soil for as many as 15 weeks. Once the e.coli bacteria infects a human being, the person will experience diarrhea and abdominal pain, both from the bacteria

Escherichia coli O157:H7 has been found as one of the most common causes of both outbreaks of human diarrheal disease in North America and throughout the world. Major outbreaks happened over the world, and 70% to 80% of sporadic cases of classic hemolytic uremic syndrome (HUS) reported in Canada, the United Kingdom, Germany, Belgium, the Netherlands and Japan have been found associated with E coli O157:H7 infections (Boyce, Swerdlow et al. 1995). Also the other diseases including mild diarrhea, hemorrhagic colitis, and thrombotic thrombocytopenic purpura. The United States Centers for Disease Control and Prevention (CDC) estimates that E. coli causes approximately 73,000 illnesses and more than 60 deaths per year in the United State. (Woodward 2002) The cost of treatment for infections caused by consume VTEC contaminated produce is about $38 billion in average per year in the US due to the increase of foodborne illness associate with produce.

This experiment is about bacterial growth. We will demonstrate a bacterial growth curve using a closed system. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. We also used different methods to determine bacterial growth as well as a few different calculations. One way of receiving data is by using a spectrophotometer where we will record the absorption at a given time to create the bacterial growth curve. We also used the plate count method after performing a serial dilution to calculate the actual cell density at different times given. By using this method we can count the population number of the same given and see the maximum cell density

Escherichia coli, is the most prevalent pathogen which is commonly known for its affiliation with human and animal infections and diseases such as diarrhoea, in addition to several clinical symptoms including haemorrhagic colitis, haemolytic uremic syndrome (HUS), and thrombocytopenic purpura (Liu et al., 2012; Vanaja et al., 2010; Tomat et al., 2013). HUS in humans, is caused by a predominant type of enterohaemorrhagic E. coli (EHEC) in the United States. It is also the chief cause of acute renal failure in children. There has also been documentation of strings of outbreaks since its identification as a pathogen in 1982, happening basically in Canada, Japan, United Kingdom and United States, implicating domestic animals such as sheep,

In this experiment, the gram negative bacterium Escherichia coli is being subjected to various environmental factors that affect the rate of growth. These factors scrutinized were the different types of nutrients, the intensity of aeration, or the temperature at which it was stored. The purpose of this lab is to determine which factor affects the Escherichia coli the greatest. It is known that these abiotic factors affect the rate of growth the greatest if they remain at the correct conditions for living.

In this lab experiment, students had to create a growth curve for E. coli. The E. coli growth curve would illustrate the progression of the population of E. coli a set time period. In this case, the growth curve depicted the population of E. coli over a 12-hour period. The growth curve for E. coli was created from the absorbance levels, the optical density(OD), recorded from the spectrophotometer.

The purpose of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli, Pseudomonas fluorescens, Enterococcus faecalis, Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5, 25, 37, 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species. After one week they were observed for distinguishable changes by the turbidity showing an indication of bacterial growth, or the clarity an indication of no survival.

This experiment was performed to test the hypothesis if LB nutrient broth, +pGLO and -pGLO Ampicillin, and Arabinose was placed in the E. coli plates, then there will be a significant growth in the newly transformed bacteria and it will possess the ability to glow under UV light. The measurements were recorded from the bent glass tube in each glass test tube. The transformation protocol tested for the newly possessed traits in E.coli bacteria. Throughout the experiment there were many probable reasons for failure. If the pipettes and sterile loop were not thrown out in between each use, a cross contamination could cause a miscalculation in the experiment causing the data results to fail. The hypothesis that was tested was validated due to the positive results with each experiment stating that newly transformed organisms due in fact pass on traits.