E. Coli

Three ways to assess water are positive/negative, membrane filtration and IDEXX Colilert and Enterolert assays. For membrane filtration, petri dishes and an incubator are used. For IDEXX coliert powdered medium is used for total coliform and E. Coli, while Enterolert media is used for enterococci. Also an incubator, colilert trays are used. IDEXX is more expensive than membrane filtration.

Esherichia coli also known as E. coli is a bacterium that lives in your gut. (1). it was founded by Theodore von Esherich in 1888. There are many people that can get the bacteria. Also there are thousands of strands of E.coli. Six E. coli O157 outbreaks were identified during 2007. Four of the outbreaks involved foodborne transmission. (Eshericha Coli). Six Minnesota cases and one Wisconsin case with the same or closely-related PFGE subtype of E. coli O157:H7, and an additional Minnesota case of Shiga-toxin producing E. coli that was not culture-confirmed, attended the Minnesota State Fair in August. All but one of the cases showed cattle or visited the cattle

Table 1: The area of inhibited growth of E.coli at increasing Dettol concentrations shown by the distance of clearing from the soaked disk

AIM – The aim of the experiment is to determine the relative effectiveness of several anti-microbial substances on developing pathogens. (E. coli)

(Biology Dept.). 0.1 ml of E.coli K or 0.1ml of E.coli B was added to the 10 fold dilution. Using soft agar technique, the growth media mixture with E.coli was plated and incubated.

The experimental part of the lab consists of setting up the materials needed. A sample of E.coli and a solution of calcium chloride are first obtained and placed in different test tubes. 630µL of Calcium Chloride (CaCl2) are then removed from the test tube and inserted into the test tube containing E.coli cells (Alberte et al., 2012). The newly formed substance of Calcium Chloride and E.coli is then mixed and incubated in ice for 10 minutes, making the cells more competent. Two test tubes are obtained and labeled; the first test tube is labeled with pUC18 and the second one with “Lux” to represent the plasmids being used. These two test tubes are then incubated in ice. 3µl of the set plasmid are added to each of the two test tubes. The test tubes are tapped to guarantee the cells are well

The purpose of this experiment is to make E.Coli competent so that it can be transformed in order to become immune to ampicillin, then we would be able to determine the transformation efficiency of the culture. We determine this by preparing 4 plates of E.coli, each labeled “LB-plasmid”, “LB+plasmid”, “LB?Amp-plasmid”, and “LB/Amp+plasmid”. This meant that either should have lacked plasmid and Ampicillin, with plasmid but lacked Ampicillin, without plasmid but with Ampicillin, or were with Ampicillin and plasmid, respectively. Then we made the bacterial cells competent by adding CaCl2 to 2 vials of the colony (one with plasmids), and incubating on ice, then heat shocking, and returning to ice. Luria Broth is then added and left to sit for 5-15

On August 19th, 2015 this experiment was performed, by 6 separate lab groups.The experiment began by measuring 1 Ml of E. coli into a pipette and pump, then placing the bacteria into a culture medium. The E. coli and medium were then swirled together for a period of 15 minutes, until completely mixed. This mixture was then poured into a petri dish and allowed to solidify for 45 minutes. After the 45 minute solidification time, 5 small paper disks were inserted into the dish. 4 of the disk contained treatments of antibiotic and 1 was left untreated. The

As Schlosser proves with his alluring facts, E. Coli is one of the most popular infections to be recognized throughout the nation. With his in-depth research, he provides the stories of the average everyday American who has been infected or passed away with such a horrid disease. Furthermore, Ingrid Abboud, from nittygriddy.com states, “Studies revealed 50% of fountain drink dispensers were found to be contaminated with fecal bacteria”.

Escherichia coli, or E. coli, is a common bacterium that can be found in diverse environments all over the planet, including the gastrointestinal tracts of animals and humans. Many of these strains of E. Coli are essential mechanisms in the digestive tract, while others are pathogens that can cause complications in urinary and intestinal tracts. (Payne & Sparks) In research, E. Coli is commonly used as a model organism, meaning they are widely studied by scientists for a variety of purposes due to their experimental advantages. E. Coli is comparatively simple, and there are many advantages to studying these prokaryotic cells in the fields of biochemistry and molecular biology. E. Coli has this simplicity and is relatively easy to propagate in a lab environment. Their genome has been completely sequenced and many things we know about DNA, protein synthesis, and gene linkage have been derived from studies regarding this particular organism. (Cooper)

E. coli or Escherichia coli is a prokaryotic cell found the in lower digestive track of mammals and other warm blooded animals. E. coli is an easy bacteria to work with as it doubles quickly and is relatively easy to grow, millions of cells can be grown in several hours. E. coli is an ideal bacteria in the lab because it does not require its temperature to be too hot, too cold, or too precise. A general warm temperature is perfect for this bacteria. E. coli is also easy to care for it does not need a specific type of nutrient, in a lab setting it can be feed any agar, making the bacteria over all cheaper to care for. Strains of E. coli can also operate in aerobic or anaerobic environments.

E. coli bacteria normally lies in the intestinal tract of people and animals. In most cases, E. coli is a harmless bacterium and even serves as an essential part of a healthy human intestinal tract. However, there are multiple types of E. coli and some are pathogenic, meaning they can cause illness. Pathogenic E. coli strains are categorized into six pathotypes: STEC, EHEC, ETEC, EPEC, EAEC, EIEC, and DAEC. Pathogenic forms of E. coli bacteria are able to infiltrate, grow and live in a variety of foods and beverages. Once a food or beverage is contaminated and then consumed, the individual who has consumed it is at risk of an E. coli infection.

In the first part of the experiment, we plated donor bacteria, which was chloramphenicol resistant, on a Nal plate. Because no donor bacteria would grow on a Nal plate, this was a way of ensuring that the sample of donor E. Coli bacteria was pure.

Most e coli do not affect people and actually are a very important part of keeping people healthy especially in the intestinal tract. Although there are some e coli that are pathogenic this means that they cause illness this is done either by giving people diarrhoea or illness that is outside the intestinal tract. These types of e coli that cause diarrhoea are usually transmitted through contaminated water ,food, or contact with animals or persons. E coli kill people by causing dehydration from diarrhoea and vomiting. E coli are considered gram negative bacteria. Colonies of e coli are circular in shape as well as having an entire margin it is also raised and size is considered punctiform (small). The texture of the colony is also smooth and the appearance is shiny and is no pigmented (colourless) as well as being

In this lab experiment, students had to create a growth curve for E. coli. The E. coli growth curve would illustrate the progression of the population of E. coli a set time period. In this case, the growth curve depicted the population of E. coli over a 12-hour period. The growth curve for E. coli was created from the absorbance levels, the optical density(OD), recorded from the spectrophotometer.