Effect Of E. Coli On Operons

2. Why do you think Mrs. Garner's students did not become ill, while Ms. Hines' kids did?

This pBlu lab had for purpose to present the changes of the strain of E. coli bacteria due to new genetic information being introduced into the cell. In this experiment we are freezing and heat shocking the E. Coli bacteria that is then forced to take the plasmid DNA. The E. coli then transforms the pBLu plasmid, which carries the genes coding for two identifiable phenotypes. After following the Carolina Biological steps our lab worked well and we able to see some colonies of bacteria on the plates. The x-gal plate showed a significant amount of bacteria to confirm that the pBlu plasmid took over the E. coli strain.

Esherichia coli also known as E. coli is a bacterium that lives in your gut. (1). it was founded by Theodore von Esherich in 1888. There are many people that can get the bacteria. Also there are thousands of strands of E.coli. Six E. coli O157 outbreaks were identified during 2007. Four of the outbreaks involved foodborne transmission. (Eshericha Coli). Six Minnesota cases and one Wisconsin case with the same or closely-related PFGE subtype of E. coli O157:H7, and an additional Minnesota case of Shiga-toxin producing E. coli that was not culture-confirmed, attended the Minnesota State Fair in August. All but one of the cases showed cattle or visited the cattle

Given the background, we hypothesized that for the first experiment, the lactase will break down lactose in milk and have a similar effect to sucrose. We also predicted that the Milk + Lactase reactant would have more glucose, the Milk + Water reactant would have a little bit of glucose broken down, the Sucrose + Lactase reactant would have less glucose than the Milk + Water reactant, and the Sucrose + Water reactant would have little to no glucose at all. As for the first procedure of the second experiment, we had hypothesized the more basic the solution would become, then the more glucose there would be. Our prediction for the first procedure of the second experiment was that there would be no glucose found in the solutions containing pH 4 and pH 7. For the second procedure of the second experiment, our hypothesis was that glucose would be present in the reactants at 4ºC and 25ºC while the reactant that had been in the hot water bath at 100ºC would have little to no glucose because it would have evaporated. We predicted that for this temperature experiment, the glucose would evaporate at 100ºC and would remain at 4ºC and 25ºC. For the first experiment we had found that a reactant of Milk + Lactase have high levels of glucose, while the other three reactants do not. As for the second experiment, for the first procedure, amounts of glucose were found in

E. coli is a single chromosome (haploid). E. coli has 4600 kilobases with about 4300 coding sequences and has around 1800 known E. coli proteins. 70% of its genes are monocistronic and 6% are polycistronic, roughly having the same amount of genes on each strand [2]. E. coli is a member of normal flora that inhabits humans and other animals. E. coli is heterotrophic and usually obtains its food from the organism it is living in. E. coli is dependent on carbon; its main source of carbon is obtained from glucose. E. coli is a facultative anaerobe meaning that it can use oxygen and other electron acceptors as the final acceptor in an anabolic or catabolic pathway. The optimum temperature of E. coli is 37o C,

1. EMG 9 and EMG 26 contain strain _lac-_(I- Z+ Y+) and strain _lac -_ (I+ Z- Y-)respectively.Three genes huddled together on the chromosome are required for two strains of _E.coli_ to utilize lactose.Consisting of three genes, namely, _lacZ_, _lacY_ and _lacA_, the _lac_ operon orderly handles these genes to code specific enzymes necessary for the metabolism of lactose. The genes _lacZ_, _lacY_ and _lacI_ would code for beta-galactosidase, galactosidase permease and _lac_ repressor respectively. Regulation of _lac_ operon is also tight and the operon's negative control is made possible by _lac_ repressor (Hill, 1996). With the presence of _lac_ repressor, _lac_ operon will be deactivated and will

Escherichia coli O157:H7 causes 73,000 illnesses in the United States annually. A review of E. coli O157 outbreaks reported to Centers for Disease Control and Prevention (CDC) to better understand its epidemiology. E. coli O157 outbreaks revealed that in that period, 49 states reported 350 outbreaks, representing 8,598 cases, 1,493 (17%) hospitalizations, 354 (4%) hemolytic uremic syndrome cases, and 40 (0.5%) deaths. Clinical laboratories began examining more stool specimens for E. coli O157. In 1994, E. coli O157 became a nationally notifiable infection, and by 2000, reporting was mandatory in 48 states. Escherichia coli O157:H7 was first recognized as a pathogen in 1982 during an outbreak investigation of hemorrhagic colitis.

The enzyme that will be used in this experiment is lactase. Lactase is an enzyme that breaks down a sugar found in milk called lactose into glucose and galactose. Lactase is found in the small intestine, and is naturally produced by the body. If someone’s body does not produce the lactase properly, or the lactase itself doesn’t act properly, that person would be considered lactose intolerant. This means your body would not be able to digest milk properly, and you could suffer symptoms such as cramping, diarraea, painful gas, and nausea after having some sort of dairy. If one would was lactose intolerant, but wanted to have some sort of dairy product, they could take a lactase supplement. The lactase in the supplement will break down the lactose

When lactose becomes available the genes encoding β-galactosidase and lactose permease are upregulated in E. coli.

Escherichia Coli, located in one of the main organs known as the large intestine, is a type of bacteria that helps digestion. (Trzepacz, Timmons, and Duobinis-Gray, 2016) For Escherichia Coli to remain stable and alive, it needs specific necessities. These needs are known as the following: Energy, vitamins, and similar compounds. Escherichia Coli is found everywhere!

E. coli is a bacterium capable of being transmitted from one person to another. To ensure that the bacteria used in this experiment were not transmitted to other places, items, or organisms, several safety protocols were followed.

At the end of 2006 both Iowa and Minnesota suffered an out break of E.Coli from Taco Johns. The restaurants that were affected were in Cedar Falls, Albert Lea, and Austin. In December of 2013 Iowa health officials recorded that at least 50 of its residents became ill with E. coli and 27 from Minnesota. January of the following year the Food and drug administration stated that the FDA was close to identifying the source of the E. coli break out. According to the FDA 81 individuals became ill. 26 of those ill were hospitalized with E. coli infections and two even developed hemolytic uremic syndrome.

Escherichia Coli, or E. coli, resides in the large intestine where it assists digestion and make up essential molecules like Vitamin K. For the most part, E. Coli poses no threat although there are few disease causing strains that can cause mild to severe food poisoning by finding its way into food supply (Timmons, Trzepacz, Duboinis-Gray). Enteropathogenic Escherichia coli, or EPEC, is a strain of diseasing causing E. Coli that is the leading cause of death in children with diarrhea in many parts of the world. EPEC colonizes in the intestine allowing the host to be invaded which can spread from person to person (Pearson, Wong fok Lung, Hartland, Giogha). Another strain of disease causing E. Coli also causes diarrhea in children is the DEC

Escherichia coli, commonly known as E. coli, is a bacteria. Though most types of E. coli are not harmful, there are certain pathogenic varieties. In shape, E. coli is a rod shaped (bacilli,) and about 1-2 micrometers in size. On the outer membrane, they have flagellum which they use for movement, and fimbria. Fimbria is slightly shorter than flagellum and is known as the “attachment pill,” as it is what the bacterium uses to attach to the host organism, in this case mostly the inside of an organism’s intestines. On the inside, E. coli has cytoplasm and DNA.

E. coli is has become a highly characterized organism across biological studies as a whole and it has also begun to be adapted in synthetic biology for the purpose of cancer therapeutics. Collaborative efforts