Osmosis Egg Lab

Cells and molecules in the environment are constantly moving and changing, for cells to function properly there is a need for equilibrium to be met. The size of the cell and the solution outside of the cell affects the rate of diffusion and osmosis in the cell. Cells are constantly trying to reach an equilibrium with the molecules and substances around it, which is why there are such terms as: hypertonic, hypotonic and isotonic. The procedures allowed testing of whether or not surface area or volume increased diffusion and how different substance control diffusion. Cells are constantly moving to reach equilibrium through diffusion and osmosis.

In the rubber egg lab also known as the osmosis lab, I first measured the circumference of the egg which was 6 inches. The egg had no cracks and was hard. I placed the egg in the vinegar and within seconds the egg started to bubble. These bubbles were carbon dioxide. After 72 hours the shell of the egg had started to dissolve or flake. This would be considered passive transport as the vinegar diffused across the egg shell without any force. Seventy-two hours into the experiment the membrane was exposed, and had a circumference of 8 inches. Before the egg had a shell and you couldn’t see through it but once the vinegar acted as an acetic acid it broke down the shell and left a yellow membrane that felt like rubber this is an example of diffusion. I then placed the egg in another container and put corn syrup over the egg for 24 hours. The egg had shriveled and shrunk, the water had left the egg and went into the syrup and that is what caused the egg to shrink. The corn syrup is essentially pure sugar with very little water so the osmotic pressure is very low. I then placed the shriveled egg in water and waited for another 24 hours. After observation the egg had no

Osmosis took place in the egg under a variety of conditions because the membrane of the egg has tiny openings where water can pass through from high to low concentrations. On day one, it was a hypotonic solution because the higher concentration of water in the vinegar moved to the smaller concentration in the egg. This means the egg was filled with water, which resulted in the eggs increase in size. On day three, the hypotonic solution occurred because the corn syrup had a lower concentration of water than the egg. That means the egg released water into the corn syrup, which resulted in the eggs decrease in size. On day four, the egg was placed in water which resulted in an isotonic solution where the concentrations become even. This

In all living organisms, water and nutrients make their way to tissues and cells because of osmosis, which for all intents and purposes is essential to the survival of cells (Rao & Kaur, 2007). Besides, cells and by extension organisms also get rid of their waste products by osmosis. In animals, for instance, this process occurs in renal tubules (kidneys) that filter and clean the blood to get rid of toxic metabolites and other chemicals. In order to understand how cells acquire and get of nutrients, it is imperative to know how osmosis operates. The objective of this experiment is, therefore, to study six decalcified eggs to understand and demonstrate the process of osmosis between the inside of the egg’s membrane and multiple environments of water/solute (solutions) of different concentrations: deionized water, 20% sucrose, and 40% sucrose.

The osmolarity of a solution is the concentration measured of a solute. To determine the concentration of a solution, one must first understand what osmosis is and how it works. Osmosis is the spontaneous passage or diffusion of water or other solvents through a semipermeable membrane according to the Britannica Encyclopedia. The semipermeable membrane is one that blocks the passages of dissolved substances such as solutes. This process, in more recent times, provides a more accurate study of how water can diffuse across a cell membrane when water molecules have a high concentration to an area in which they have a low concentration. It was originally studied in 1877 by plant physiologist, Wilhelm, Pfeffer. (Britannica Encyclopedia 2015).

Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.

The Osmosis and Diffusion lab was conducted to provide us with information on how built up mucus affects those conflicted by the recessive genetic disease, Cystic Fibrosis., due to a mutation to the membrane regulating chloride (Cl-). This mutation prevents the Cl- from leaving the cell causing the amount of sodium (Na+) in epithelial cells, which results in extreme mucus on the lungs and airways causing this disease to be fatal if not treated but treatment does not equate to a long lifetime. During the lab we took the data from three parts: Diffusion, Osmosis in an Elodea Cell, and finally the Role of Osmosis in Cystic Fibrosis. During Part 1 we looked at diffusion across a semipermeable membrane for starch and glucose, which resulted in both having a negative solution when placed in a semipermeable membrane. Then we looked at osmosis in the Elodea Cell to watch for the occurrence of Plasmolysis, when a cell’s plasma membrane pulls away from the cell, and how a plant cell is affected by both hypertonic and hypotonic solutions. Finally, we observed the role of Osmosis in Cystic Fibrosis using dialysis bags to represent a normal cell and a Cystic Fibrosis cell with the normal containing 1% NaCl while the Cystic Fibrosis bag contained 10% NaCl. After we ran the experiment, we looked at the Percent Change in Mass and compared them after 30 minutes. We found that Cystic Fibrosis cells didn’t change mass as much as the normal cell ending with a change in mass over -1%. The

What will happen to the egg when a student tests osmosis with 3 different soultions in a cup.The student is trying to see or find the effects of osmosis on an egg.Osmosis is the movement of water molecules through a selecfively permeable membrane. Diffuision is when something spreads more widely and equilibrium means when somethings is at rest due to equal opposite forces. The student wants to find out if the egg will shrink, fill up, or change in any way.

The purpose to this experiment was to study the effect of osmosis in de-shelled chicken eggs in different percentages of sucrose solutions. Osmosis is the process, in which, water moves across a differentially permeable membrane. The eggs were soaked in vinegar to remove the outside hard shell but still leave the egg in its membrane. By placing the six de-shelled in different sucrose solutions, we tested the rate of osmosis. The eggs were placed in the solutions for an hour and weighed in fifteen minute intervals. Then, each weight was recorded and graphed. The results showed that the egg in the water solution gained the most weigh and the only other egg that gained a little weight was the one in the 10% solution. All the other eggs in the different solutions lost weight, even the unknown solution. According to the results the egg that was in the distilled water solution gained weight because it is the hypertonic solution. All the other eggs lost weight because they were placed in hypotonic solutions with different concentrations of sucrose. The egg that was placed in the higher concentration of sucrose lost the most weight. So, the higher the concentration of sucrose, the more water the egg lost.

When the final mass was measured the excess water in the potato cube might have not been completely removed, thus it adds to the final mass of the potato cubes. This acts as a major error because it gives an inaccurate representation of the actual percentage change in mass of the potato cubes, also while measuring the mass of each cube of potato; the value fluctuated and was not consistent.

Osmosis is the passive movement of water from an area of low solute concentration to an area of high solute concentration, normally across a membrane which prevents the movement of solvent. This is a process by which materials may move into, out of, or within cells. Osmosis doesn’t depend on energy provided by living organisms but is affected by the properties of the cell membrane. The rate of osmosis is dependent on such factors as temperature, pressure, molecular properties such as size and mass, and the concentration gradient. In osmosis, the relationship between a solute’s concentration outside of cell and inside of a cell is described in terms of the tonicity of the solution outside of the cell. A cell is in a hypotonic solution when the solute is more concentrated inside the cell and therefore water moves into the cell. In this solution the cell swells as water enters, this may continue until it ruptures or hemolyzes. In the reverse condition, the cell is in a hypertonic solution

An egg has a semi-permeable membrane, thus processes like osmosis could occur. Osmosis is the movement of water molecules from an area of higher water concentration to an area of a lower water concentration. Osmosis is important, especially for living organisms, as they help distribute nutrients in the body. An egg’s mass would change when it is soaked in different substances. The goal of this experiment was to investigate how an egg changes through osmosis. This experiment was done to show how substances affect the mass of an egg. To start off, an egg’s mass was measured by putting the egg in a beaker then placing it on a mass scale. The beaker was then filled with vinegar and left alone for twenty-four hours. After a day, the egg was taken

Osmosis is defined as the tendency of water to flow through a semipermeable membrane to the side with a lower solute concentration. Water potential can be explained by solutes in a solution. The more positive a number is more likely it will lose water. Therefore should water potential be negative the cell the less likely it will lose water. In using potatoes the effects of the molarity of sucrose on the turgidity of plant cells. According to Clemson University, the average molarity of a White potato is between .24 M and .31 M when submerged in a sorbitol solution. This experiment was conducted with the purpose of explaining the relationship found between the mass in plants when put into varying concentrations of sucrose solutions. Should the potatoes be placed in a solution that contains 0.2M or .4M of sucrose solution it will be hypotonic and gain mass or if placed in .6M< it will be hypertonic and lose mass instead. Controlled Variables in this lab were: Composition of plastic cups, Brand of Russet Potatoes, Brand of Sweet Potatoes and the Temperature of the room. For independent variable that caused the results recorded it was the different Sucrose concentrations (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M). The dependent variable was the percentage change from the initial weighs to the final. The cup with .4 molarity was the closest to an isotonic solution and was used as the control group for the lab. Water potential is the free energy per mole of water. It is

This is the final step of the experiment. The shell-less egg was now placed into a new cup, but very carefully, as the egg was more fragile than when it was soaked in vinegar. The egg in the new cup was then covered with water. The egg sat in the water for a 24 hour time period once again. During this 24 hour time period the eggs appearance and size were once again recorded. After the time period had completed, the egg was removed from the water and was very carefully patted dry. Since the egg was now swollen, extra care was needed when holding the egg. The swollen egg was placed on the scale on last time and its mass was recorded as M3.

In conclusion, the data that was collected helped tell us that the solutions greatly affected the cell of the egg because it caused the egg’s mass to increase than decrease. Also saw how the solutions played a role in the phospholipid bilayer and saw how it altered the shape of the egg. However, it does not support my hypothesis on the affect the egg would undergo onced placed in sugar and water but sugar was not used in this experiment, but water was used and it didn’t affect the shape of the egg, all it did was cause the egg to be in a isotonic state. According to Khan Academy (2017), states that a cell is isotonic “When the extracellular fluid has the same osmolarity as the cell, there will be no net movement in or out of the cell”. Which