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Effect Of Inhibition On Bacteria

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Hypothesis
The antibiotics will have different zones of inhibition on different bacteria. Also, a range of concentrations of the nutrients in the bacteria will change the bacterial growth.
Background information
Bacteria affected 2 million people from antibiotic resistance bacteria and 23,000 die per year due to the infections. Bacteria works by entering the body in various ways, they can enter past nose, mouth or ears as they are small, they can be inhaled from the air, eaten from food as well as through cuts/ open wounds.
Bacteria enters the body and asexually reproduces, they release toxins which cause infection.
Antibiotics either stop the bacterial cell from reproducing or kill the cell. They can disrupt the bacteria by deterring …show more content…

Ampicillin, penicillin, streptomycin all sulphafurazole all were resistant to the bacteria as it may have grown a mutation.
Most effective Erythromycin Chloramphenicol Tetracycline
Least effective Cefoxitin

Bacillus Subtilis only had one antibiotic which had was resistant, sulphafurazole. But all the other antibiotics had zones of inhibition. Theses antibiotics were effective and can be a choice for treating B.subtilis.
Most effective Streptomycin Tetracycline Chloramphenicol Erythromycin Cefoxitin …show more content…

Although some agar plates were hard to see if the streptomycin had a definitive zone of inhibition. Ampicillin, erythro-mycin, penicillin, sulphafurazole was ineffective with no inhibition zone.
The trend I saw in the different concentrations of nutrients was the rod shaped bacteria which be-come more obvious when more nutrients are added. At 0.1g, the agar plate looks mostly smooth but at the 0.6g individual rod, shapes are prominent. Once it gets to 1.3g the colonisation is more crowded, making it harder to see. This seems only visible effect of altering the nutrients is the rod shaped forming and the visibility of them.
Graphs- appendix
Evaluation

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