Effect of Concentration on Enzyme Activity Lab Report

608 Words3 Pages
Lab Report
(Effect of concentration on enzyme activity)
Biology
Noor Alawadhi
11- KC

Introduction:
An Enzyme is a protein, which is capable of starting a chemical reaction, which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives, grows, or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of substrate on the enzyme’s reaction.

Research question:
What does the changes in the amount of substrate on an enzyme’s reaction effect on?

Hypothesis:
I forecast that the more concentrated the hydrogen peroxide is the higher the volume of
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Make sure you rinse your glassware carefully between procedures. Repeat this procedure five times. 5. Use this data to calculate the rate of reaction of this enzyme 6. Plan and conduct your own investigation into one other factor that affects the activity of catalase, you may use this as the basis for your investigation but if it is to be for moderation of internal assessment you must modify it substantially.

Results: Different type of hydrogen peroxide | Volume of foam cm³ | 0.5% | 5 cm³ | 1% | 7 cm³ | 2% | 13 cm³ | 4% | 21 cm³ | 6% | 24 cm³ |

The volume of the foam using different concentrations of hydrogen peroxide:

Conclusion:
As I forecasted the results proved correct that, the more concentrated the hydrogen peroxide is the higher the volume of the foam is and the less concentrated the hydrogen peroxide is the lower the volume of the foam will become. Other than the table of results the graph is also evidence to my true hypothesis. Each time the concentration of hydrogen peroxide doubles the level of foam increases but the more the concentration the foam reaches its constant point therefore slowly increasing. An example is when the 2% concentration of hydrogen peroxide was added the foam level was 13cm³ but when 4% was added to the next trial the level of foam greatly increased to 21 cm³ and as it reached its constant point, when 6% it slowed down and increased only 3 cm³ more which became 24 cm³.

Evaluation:

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