Effect of Lead Ions On Bacterial and Fungal Amylase Essay

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Effect of Lead Ions On Bacterial and Fungal Amylase Amylases are enzymes, which hydrolyse starch into Maltose. There are two types of amylase: Alpha amylase that degrades starch, which is a polysaccharide, into fragments 10 glucose residues long. Beta Amylase breaks these down into maltose, which is a disaccharide of two glucose molecules joined together. Both enzymes work by hydrolysis of the glycosidic link in carbohydrates. Enzyme amylase are produced from different sources but in my experiment I will be concerned with Bacterial and fungal amylase. Heavy metal ions cause inhibition of enzyme. Inhibition of an enzyme means either to slow down the rate of reaction, to increase the rate…show more content…
So in order to see the effect of lead ions into the enzyme rate of reaction it will depend on the concentration of lead molecules and where the inhibitor binds the active site. My hypothesis ============= The rate of enzyme reaction would certainly decrease with increased concentration of lead ions. Lead ions are likely to have different effect on bacterial and Fungal Amylase. If lead ions temporarily bind onto the active site then increased concentrations more inhibitors would join more frequently preventing the action of limited substrate concentration there would be. If lead ions act as non-competitive inhibition then with increased concentration there will be point where no substrate will be able to access the active site so the enzyme will not be able to function. My prediction graph will be able to the rate of reaction with increased concentration of lead ions Inhibitions are likely to react differently with different types of enzyme so the rate of reaction of both enzymes would be different. One difference would be size of active site enzyme could lead to have faster rate of reaction in Fungal amylase compared to Bacterial amylase. Different results in the reaction of both enzymes would enable to find out the answer by looking at their protein structure. Bacteria and fungi due to different conditions

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