Rate Of Diffusion Lab Report

2. Does the rate of diffusion correspond with the molecular weight of the dye? The the density of the medium and the molecular weight of the dye will determine the rate of diffusion.

Cells and molecules in the environment are constantly moving and changing, for cells to function properly there is a need for equilibrium to be met. The size of the cell and the solution outside of the cell affects the rate of diffusion and osmosis in the cell. Cells are constantly trying to reach an equilibrium with the molecules and substances around it, which is why there are such terms as: hypertonic, hypotonic and isotonic. The procedures allowed testing of whether or not surface area or volume increased diffusion and how different substance control diffusion. Cells are constantly moving to reach equilibrium through diffusion and osmosis.

2. Explain your observations in detail in terms of concentration gradient, diffusion, osmosis, osmotic pressure, passive transport, and active transport.

After the serial dilutions of the red and blue dyes were taken, the molarity and absorbance for both dyes were calculated. Using the MiVi = MfVf equation, the concentrations for each value of the red and blue dye were separately calculated. Calculating absorbances calls for setting the correct wavelengths of light for each dye. In this case, the 470 nm wavelength for red dye and the 635 nm wavelength for blue dye was needed to find the maximum absorbances. The absorbance was found by blanking the colorimeter and entering the concentrations. After both values of the absorbances and concentrations were found, the values were then graphed in order to obtain the equation of the relationship between absorbance and concentration.

Aim: To plan and carry out an investigation to show the relationship between volume/Cm3, surface area, and diffusion using agar cubes measured in time/s; and to demonstrate, using diffusion, why the size of cells is limited, keeping the room temperature and pressure constant.

All organisms need to exchange particles such as food, waste, gas and heat with their surroundings. there are two different types of ways in which substances can enter or/and leave a cell. these ways are called Passive and Active. with in these there are different processes for example, in passive process there are diffusion, facilitated diffusion and osmosis and in the active process there is molecule and particle. in this assignment I am going to discuss all of these processes starting with diffusion.

7. Explain how incubation plant tissues in a series of dilutions of sucrose can give an

In performing the proper experimentation, we poured some of the methylene blue substrate in a piece of paper, for ease transport towards the agar plates. We performed it cautiously by not inhaling it, for it is a form of powder. We carefully place the substrate at the center of the agar plates, to ensure that is that there is enough space for diffusion to take place. After the moment we placed the substrate, we carefully placed a transparent ruler under the plate to measure the diameter of the substrate in 0 minutes. We carefully note the time, having a 15-minute interval and record the changes in the diameter of the substrate. We continued it, until we have an hour observation.

The difference is that along with large molecules, living cells prevent molecules with positive charges and solubility. This is not representing in dialysis tubing, and is only found in living cells because the tubing is only based on molecular size (98). When referring the rate of diffusion, the concentration gradient influences the diffusion rate, based on the factors of temperature. The ability for molecules diffuse from high to low concentrations primarily depends on the concentration gradient between the two areas.(96-99). My hypothesis for the study is that in the hypotonic, hypertonic, and isotonic solutions, the direction and rate of osmosis will determine based on the concentration inside the dialysis tubing. My prediction is that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant.

The objective of this experiment is to develop an understanding of the molecular basis of diffusion and osmosis and its physiological importance. Students will analyze how solute size and concentration affect diffusion across semi-permeable membranes and how these processes affect water potential. Students will also calculate water potential of plant cells.

In order to assimilate diffusion through a permeable membrane potassium permanganate and methylene blue were used in experiment. The objective was to compare the rates at which the liquid compound of different molecular weight diffused through agar. This was achieved by obtaining agar in a petri dish with two wells to hold the liquid compounds. The rate was measured by time and diameter distance diffused. This process was observed for 60 minutes at 15 minute intervals.

In conclusion, the potato cube with the highest surface area to volume ratio (the 1x1x1 cube) had the fastest rate of diffusion as it had the largest percentage increase in mass. While all the other cubes of potato had larger increases in mass at face value compared to the smallest cube, the smallest cube had the largest overall gain in percentage. The results support my hypothesis that the smallest cube will have a higher rate of osmosis because it has a proportionally larger amount of surface area compared to its volume.

Diffusion is the transfer of molecules from an area that has a higher concentration to an area that has a lower concentration. Osmosis is the diffusion of water. The purpose of this experiment was to study the process of osmosis. In order to test osmosis, eggs that had been soaking in vinegar were taken and placed in four beakers of solution with different levels of glucose. Using this experiment we were able to determine the rate of osmosis of different solutions, with various amounts of glucose, through eggs. In the results of this lab it was found that the eggs were either hypertonic or hypotonic and that the

The diffusion across a cell membrane is a process of passive and spontaneous net movement of small lipophilic molecules. The molecules move from a high concentration to a low concentrated region along the concentration gradient. The result being a point of equilibrium, this is where a random molecular motion continues but there is no longer any net movement. However, there are things that can affect the rate of diffusion, these being temperature, surface area, concentration, size of the molecule, permeability, diffusion distance and concentration difference. Osmosis is a type of diffusion as it is the movement of water molecules through a semipermeable membrane into a region of higher solute concentration. Equilibrium is reached when the solute concentration is equal on both sides. Water potential is measured in kiloPascals, it is the measuring of the concentration of free water molecules that are able to diffuse compared to pure water, which is 0 kilopascals. It is a measure of the tendency of free water molecules to diffuse from one place to another. The result being, the more free water molecules, the higher the Water Potential. However, Water potential is affected by two factors: pressure and the amount of solute.

Cells are always in motion, energy of motion known as kinetic energy. This kinetic energy causes the membranes in motion to bump into each other, causing the membranes to move in another direction – a direction from a higher concentration of the solution to a lower one. Membranes moving around leads to diffusion and osmosis. Diffusion is the random movement of molecules from an area of higher concentration to an area of lower concentration, until they are equally distributed (Mader & Windelspecht, 2012, p. 50). Cells have a plasma membrane that separates the internal cell from the exterior environment. The plasma membrane is selectively permeable which allows certain solvents to pass through