Effect of Solute Concentration on Osmosis in Potato Cells Report

Aim: To find the concentration of a potato cell cytoplasm compared to sucrose and salt. I mainly was looking to see how concentration of salt/sucrose affects the mass of the potato cores.

I know that osmosis will occur in the vegetables, but I am not sure of

Osmosis is a natural occurrence constantly happening within the cells of all living things. For osmosis to occur, water molecules must move across a semipermeable membrane from an area of low concentration to an are of high concentration. In order to understand osmosis, people must understand the different types of concentrations that can be present within solution. One of them is an Isotonic solution where the concentration of dissolved particles is equal to that of a cell’s. Another is a hypertonic solution where there is a higher concentration of dissolved particles then inside the cell. And lastly there is a hypotonic solution where there are less dissolved particles than inside the cell. As dissolved particles move to a region of lower concentration, water moves the opposite direction as a result of there being less water in the highly concentrated region. In this experiment, gummy bears were placed in salt water, sugar water, and tap water to find the measure of osmosis between the solution and gummy bear.

The lab for this paper was conducted for the topic of osmosis, the movement of water from high to low concentration. Five artificial cells were created, each being filled with different concentrated solutions of sucrose. These artificial cells were placed in hypertonic, hypotonic, or isotonic solutions for a period of 90 min. Over time, the rate of osmosis was measured by calculating the weight of each artificial cell on given intervals (every 10 minutes). The resulting weights were recorded and the data was graphed. We then could draw conclusions on the lab.

Osmosis is the diffusion of water across a membrane to create an equilibrium between the levels of concentration of a solute both inside and outside the cell. In this case the solute will be sugar as the potato core will be immersed in sucrose solution.

Water diffuses across the membrane from the region of lower solute concentration (higher free water concentration) to that of higher solute concentration (lower free water concentration) until the solute concentrations on both sides of the membrane are equal. The diffusion of free water across a selectively permeable membrane, whether artificial or cellular, is called osmosis. The movement of water across cell membranes and the balance of water between the cell and its environment are crucial to organisms. ("Diffusion And Osmosis - Difference And Comparison | Diffen"). A semi-permeable membrane known as the cell membrane surrounds the living cells of both plants and animals. Both solute concentration and membrane permeability are

Osmosis is defined as the tendency of water to flow through a semipermeable membrane to the side with a lower solute concentration. Water potential can be explained by solutes in a solution. The more positive a number is more likely it will lose water. Therefore should water potential be negative the cell the less likely it will lose water. In using potatoes the effects of the molarity of sucrose on the turgidity of plant cells. According to Clemson University, the average molarity of a White potato is between .24 M and .31 M when submerged in a sorbitol solution. This experiment was conducted with the purpose of explaining the relationship found between the mass in plants when put into varying concentrations of sucrose solutions. Should the potatoes be placed in a solution that contains 0.2M or .4M of sucrose solution it will be hypotonic and gain mass or if placed in .6M< it will be hypertonic and lose mass instead. Controlled Variables in this lab were: Composition of plastic cups, Brand of Russet Potatoes, Brand of Sweet Potatoes and the Temperature of the room. For independent variable that caused the results recorded it was the different Sucrose concentrations (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M). The dependent variable was the percentage change from the initial weighs to the final. The cup with .4 molarity was the closest to an isotonic solution and was used as the control group for the lab. Water potential is the free energy per mole of water. It is

out the potato and dry it to ensure excess water is not added to the

Van’t Hoff’s Law suggests that the osmotic potential of a cell is proportional to the concentration of solute particles in a solution. The purpose of this experiment was to determine if there are any differences between the osmolalities, the no-weight-changes of osmolalities, and the water potentials of potato cores in different solutions of different solutes. The percent weight change of the potato cores was calculated through a “change in weight” method. The potato core’s weight was measured before and after they were put into different concentrations of a solute for 1.5 hours. In our experiment, there were no significant differences from the osmotic potentials of our results and the osmotic potentials of other scientists work. Ending with chi square values of 2.17 and 2.71, and p values of 0.256 and 0.337, concluding that there is no difference in water potentials of potato cores in different solutions of different solutes at varying concentrations.

Group 1 received 107% for 0.0m, 109% for 0.25m, 112% for 0.5m, and 126% for 0.75m. Group 2 received 100% for 0.0m, 107% for 0.25m, 116% for 0.5m, and 117% for 0.75m. Group 3 received 100% for 0.0m, 106% for 0.25m, 114% for 0.5 m, and 117% for 0.75m. Group 4 received 100% for 0.0m, 106% for 0.25m, 113% for 0.5m, and 116% for 0.75m. Group 5 received 98% for 0.0m, 105% for 0.25m, 110% for 0.5m, and 115% for 0.75m. Group 6 received 104% for 0.0m, 108% for 0.25m, 111% for 0.5m, and 104% for 0.75m. The trend line that occurred for the rate of osmosis was an upward increase as the solute concentration increased.

Effect of Sucrose Solution on Osmosis Aim: The aim of the experiment is to show how varying the concentration of sucrose solution affects osmosis by changing different molar solutions of sucrose and water and how it affects the potato. Introduction: In this investigation I will be exploring the effect of varying concentration of sucrose sugar solution on the amount of activity between the solution and the potatoes. Osmosis is the movement of water molecules across a partially permeable membrane from a high water concentration to a low water concentration.

The potatoes were placed into the saucers for 45 minutes and observed. Then, the potatoes were placed into the opposite saucer for another 45 minutes and observed.

In conclusion when the molarity level was at 0 and at .2 the potatoes had gained mass so therefore they were placed in a hypotonic environments. When the molarity level was .4 and above the potatoes loss mass so therefore they were placed in hypertonic environments. So the different in concentrations does change the mass of the potatoes because they determine the osmosis environments.

The purpose of this experiment was to investigate the effect of the change in concentration of sucrose on the rate of osmosis in cylinders of potatoes.

Osmosis is when water passes through a cell membrane, it is also form of a diffusion, which is a form of passive transport. Osmosis will continue to until an equilibrium is reached which is when the solutions are isotonic. This means that the solution has the same amount of solute on both sides. If the solution is hypertonic, it has more solute in the solution. In this situation water will move towards it. if the solution is hypotonic, it has less solute in the solution. Whereas in this situation, water will move out of the solution.