Effect of caffeine, nicotine, alcohol, epinephrine on Daphnia

According to the results, the columns of caffeine in figure 1, of this experiment the hypothesis for caffeine is partially accepted. There is an increasing trend in the change of pulsation rates with increasing

After completing the experiment we found that when we gave the Daphnia caffeine the heartbeat rate did show an increase. However, we also found that alcohol also increased the number of times the heart beat. Even though we performed all of the experiments very carefully, we cannot be certain that the effect we saw was due to the drugs. Perhaps the change in heartbeat rate is caused by

1). Another study shows that nicotine stimulates the adrenal medulla to secrete hormones such as epinephrine and norepinephrine which are responsible for the increase in the pulsation rate (George, "Effects of smoking on cardiovascular function: the role of nicotine and carbon monoxide", 2015, pp. 278-280).

Resting heart contractions were recorded for thirty seconds until the heart rate was less than 60 beats per minute. A stimulator electrode to be used was set to the following states: Amplitude of 4.00 Volts, a stimulus delay of 50ms, stimulus duration of 10ms, a frequency of 1.0Hz, and a pulse number of 30. The electrode was then placed in direct contact with the heart for 30 seconds at which time the data was observed and recorded.

The respiration rate for the control goldfish ranged from 123 to 140 breaths per minute, which was not a significant change. On average, the cold-water treatments caused a significant decrease in breaths per minute by the end of the experiment. The average the breathing rates of goldfish subjected to temperatures less than 22°C decreased from a rate of 96 breaths per minute at the start of the experiment, to 56 breaths per minute at the end (Figure 1). The experimental fish in Group #1 ranged from 115 to 50 breaths per minute. Overall, the control fish’s breath rates generally remained constant, and the temperature-stressed goldfish had rates that decreased rapidly from start to finish.

This laboratory exercise was performed to visualize the effects of various drugs on the heart rate of Daphnia magna. The four drugs tested consisted of: Lidocaine, Acetylcholine, Caffeine, and Nicotine. These drugs were designed to have an apparent effect on the average heart rate of the Daphnia. The laboratory exercise was divided into two parts and procedures: measuring the basal heart rate of Daphnia, and measuring the drug induced heart rate of Daphnia. In order to measure the basal heart rate, various Daphnia were obtained and observed under a microscope at 40x magnification for two separate trials. Using the same technique, the drug induced heart rate with each drug (Lidocaine, Acetylcholine, Caffeine and Nicotine) was measured and

5. Establish the resting heart rate of the Daphnia. Count the amount of beats for ten seconds then multiply that number by six to convert it to beats/min.

To conduct this study a culture of living Daphnia were collected from a nearby natural water source area.

To conduct this study a culture of living Daphnia were collected from a nearby natural water source area.

In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.

My hypothesis was, as the concentration of alcohol increases the lower the heartbeat of the Daphnia will fall. To test my prediction I carried out 25 individual experiments which were all carried out using the same procedure but varying the alcohol concentration of the solution. To investigate how alcohol concentration affects the heartbeat of Daphnia I recorded the heartbeat of 25 Daphnia for a period of 7 minutes. This included 1 minute before submersion in alcohol, 3 minutes during submersion in alcohol and 3 minutes after submersion in alcohol. I then found the percentage decrease of the heartbeat of the Daphnia from the resting heart rate.

An Experimental and Statistical Analysis of SDH activity as compared in Liver, Kidney, and Heart Homogenates of the Bos taurus

The heart serves an important purpose within the body, pumping blood throughout the circulatory system to supply all parts of the body with vital nutrients and molecules. It pumps oxygen and nutrient rich blood to be exchanged for carbon dioxide, which is then pumped to the lungs and eliminated from the body. The movement of blood throughout the body is due to the heart’s ability to push blood along the circulatory system at a steady, unfaltering rate. This rate, known as heart rate, is regulated and can be altered at a moment’s notice by signaling within the body and heart itself. In vertebrates, the autonomic nervous system controls and regulates heart rate. The autonomic nervous system is divided into two subunits, the sympathetic nervous system and parasympathetic nervous system. The parasympathetic nerve that innervates the heart is the vagus nerve. In this laboratory experiment, the regulation of heart rate was observed by studying a certain breed of turtle, the Red-eared Slider (Trachemys scripta elegans). Both chemical and electric signaling can influence the components of the nervous

In the presence of excess T3 and T4, an increase in number as well as affinity of beta-adrenergic receptors in the heart is noted. These receptors facilitate the action of "fight, flight and fright" hormones ( epinephrine, norepinephrine) which are positively chronotropic ( beat faster) and inotropic ( pump harder) to the heart. This may then lead to cardiac failure in older patients.

The intention of the experiment was to measure how different pH levels affect the heart rate of daphnia. The objective of this experiment was to measure the heart rate of