Effects Of Concentration On The Rate Of Reaction

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Michaelis – Menten and Lineweaver – Burke kinetics of polyphenol oxidase . Effect of substrate concentration concentration on the rate of reaction” Introduction Enzymes catalyse biological reactions ,increasing the rate of chemical reactions Enzymes have an affect on the structure of chemical transformation (stryer et al , 2011 ) Enzyme substances bind at the active cite which contains amino acid chains. Without catalysis reactions the process would take place slowly to produce products , enzymes are most effective catalyst (Campbell and Farrell, 2011) .A catalysed reaction is obtained by the production of a complex between e and the reactants (Montgomery et al ., 1990).The reversible reaction can be seen below: E + S ⇄ ES ⇄ EP ⇄ E + P Inhibitors Competitive inhibitors bind to the active site of the enzyme and compete with substrate concentration. Competitive inhibitors do not change the vmax velocity because high amounts of substrate can over come competitive inhibitors but rather increase km. Non – competitive inhibitors cause blockage for enzymatic reactions but allow substrate to bind. Uncompetitive inhibitors don’t bind to free energy but rather to enzyme substrate concentration. Un- competitive inhibitors work more effective with high amounts of substrate concentration (Meisenberg and Simmons , 2011). Cofactors and Coenzyme Non protein molecules are required by certain enzymes in order to participate in enzyme catalysed reactions (Devlin, 2010). Co factors are
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