Metabolism Lab Report

effected the heartbeat rate of a Daphnia. The results of the experiment were that the

Mann-Whitney U statistical testing was performed to see if the result obtained for the difference in heart rate of Daphnia before and after the addition of stimulant was significant. The statistical test for the difference in heart rate of daphnia observed after the addition of aged water is shown on table 2:

The following was the procedure used by the team that introduced chemicals into the environment of the Daphnia. First a zero reading was taken before any chemicals were introduced. The zero reading was an observation of the Daphnia’s heart rate before any substances were administered. All fluids were drawn off the slide using the corner of a Kimwipe. Then two drops of two percent alcohol solution were dropped onto the Daphnia. After a minute a heart rate reading was taken. The same procedure, including using the Kimwipe to draw off previous solution, was then used with four, six, eight, and ten percent solutions. A heart rate reading was taken after each solution was introduced.

This lab covered the effects of caffeine, nicotine, caffeine extract and nicotine extract on the pulsation rates of Lumbriculus variegatus, commonly known as blackworms. The circulatory system consists of the heart and the blood vessels that circulate blood throughout the organism’s body. Blackworms do not possess a respiratory system or a heart, thus they circulate their blood through contractions of the blood vessels. The pulsation rate was observed along the dorsal blood vessel near the posterior end of the worm to give better readings. The various drugs were exposed to the black worm and the effects it had on the pulsation rate were observed. The results found in this experiment could also relate to the effects of these

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

direct calorimetry. Furthermore, the amount of oxygen in the chamber reveals the amount of cellular respiration of the organism. While also, test the effects of decreasing oxygen, and later increasing the heat on the metabolic rate of goldfish. I hypothesize that an increase in temperature will increase their metabolic rate

This laboratory exercise was performed to visualize the effects of various drugs on the heart rate of Daphnia magna. The four drugs tested consisted of: Lidocaine, Acetylcholine, Caffeine, and Nicotine. These drugs were designed to have an apparent effect on the average heart rate of the Daphnia. The laboratory exercise was divided into two parts and procedures: measuring the basal heart rate of Daphnia, and measuring the drug induced heart rate of Daphnia. In order to measure the basal heart rate, various Daphnia were obtained and observed under a microscope at 40x magnification for two separate trials. Using the same technique, the drug induced heart rate with each drug (Lidocaine, Acetylcholine, Caffeine and Nicotine) was measured and

The effects of caffeine and alcohol on daphnia are expressive of whether these substances are harmful or beneficial to the organism. By understanding the results of this experiment, it may also be understood how these substances effect humans. In this study, one daphnia was exposed to increasing levels of alcohol, while the other was exposed to increasing levels of caffeine, each in order to test the hypothesis that when given amounts of caffeine and alcohol, the daphnia will be affected the same way a human would. The effect of each substance was measured by the daphnia’s heart rate one minute after the substance was added. Results reveal that alcohol slows the heart rate, while caffeine increases heart rate. Furthermore, caffeine shows a

Metabolism is a chemical process that converts fuel from food into energy needed for the body’s activities. For example, thinking, running and jumping etc. are all an example of the bodies activities.

to the body of Daphnia. For example, an experiment may test the effects of toxic chemicals on Daphnia, meaning that Daphnia can act as water quality indicators (Ren et al. 2009). The lack of Daphnia in certain areas signifies the presence of a toxic chemical, if the habitat meets the living requirements of Daphnia (Villegas-Navarro, 2003). Therefore, Daphnia act as indicators of environmental health as well as chemical responders reacting to potentially harmful

The purpose of this experiment was to test the affect of caffeine on the heart rate of Daphnia by observing their behavior and heart rate under a microscope when exposed to different concentrations of caffeine. Caffeine is a stimulant drug used in many energy drinks and causes large amounts of stimulatory neurotransmitters to be released. Therefore, as caffeine is a stimulant drug it was hypothesized that the Daphnia heart rate would increase immensely. Daphnia is a group of microscopic, planktonic crustaceans that are arthropods that measure 1-5 millimeters in length. Daphnia live in multiple aquatic environments such as freshwater lakes, ponds and rivers. “The development of genomic infrastructure coupled with a wide range of phenotypic diversity make Daphnia a versatile model

There were also several limitations within the experiment. Since this experiment looked into the effects of green tea and black tea, which contains caffeine, observation of varying plant defensive compounds other than the caffeine in tea can widen the extent of the results and improve the experiment. For instance, observing the effects of chamomile tea, which has a little to no level of

In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.

The experiment took place in a laboratory setting, and the first step was obtaining sixty individual Daphnia magna (that were neither adults nor tiny offspring) from a large tank in the lab. These individuals were equally divided into three groups; low density, medium density, and high density. The twenty Daphnia assigned to the low density group were split into four groups of five and pipetted into one of four tubes filled with 10mL of Chlamydomonas algae. The twenty Daphnia assigned to the medium density group were split into two groups of ten and placed into one of two tubes also filled up to 10mL with Chlamydomonas. The final twenty Daphnia were all placed into a single tube filled with 10mL of the algae. In order to avoid suffocation-related

“Metabolism is the set of chemical reactions that happen in living organisms to maintain life. These processes allow organisms to grow and reproduce, maintain their structures, and respond to their environments” (Metabolism). Metabolism breaks down the food that we eat, transforming it into energy for our bodies. Metabolism is broken down into two categories Anabolism and Catabolism, which help aid in the chemical reaction process. Specific proteins in the body control the chemical reactions of metabolism, and each chemical reaction is coordinated with other body functions (Dowshen). Metabolism is a constant process that begins when we're born and ends when we die. It is a vital process for all life not just humans, and