Effects of Temperature and Solvents on the Cell Membrane

Our original hypothesis about the effect of high temperature on beet cell membrane was correct; as temperature increases, the concentration of betacyanin increases as well. The beet root sample in temperature 70°C had a higher absorbance value and concentration of betacyanin in comparison to other samples due to the membrane damage. The experiment of lipid membranes performed by the University of Osaka concluded a similar result. They found that the membranes were stable at physiological

Campbell, Neil A., Jane B. Reese, Martha R. Taylor, Eric J. Simon. Biology 105 Taken From; Biology: Concepts & Connections (Fifth Edition) Benjamin Cumming, San Francisco, CA, 2013.

27. Heat the water to 40°C with an alcohol lamp by setting up the apparatus as shown in figure 3 below:

Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.

9. Have students get back in circle and pass the rope bucket around. Ask students inquiry based questions.

Beets also known as Beta Vulgaris, contain a pigment known as betacyanin that gives the beets its deep rich red color, it is water soluble and is stored in the vacuoles of the beet roots and stems. Because the betacyanin is a water soluble pigment is can not easily cross the cell membranes.

Wischusen, William, Jolissaint, Ann, Reiland, Jane, and Pomarico, Steven. 2012. Biology 1208/1209: Biological Laboratories for Science Majors. Hayden McNeil, Plymouth,

Once the water is at the desired temperature, place the small lamp 5cm away from the beaker and turn it on.

9) Trial E: Remove the syringe and empty the beaker. Add a Thermometer to the beaker. Add 200 mL of Room Temperature water to the beaker and heat with a Bunsen Burner until it reaches 100° C. Remove the Bunsen Burner. Repeat Steps 5 & 6.

The objective of this experiment is to develop an understanding of the molecular basis of diffusion and osmosis and its physiological importance. Students will analyze how solute size and concentration affect diffusion across semi-permeable membranes and how these processes affect water potential. Students will also calculate water potential of plant cells.

Beetroots contain Betalains which are the red pigments present in the cell vacuole. Betalains are soluble in water and they contain nitrogen. Betalains extracted from beetroot is commonly used as food dye because it is not known to cause any allergic reactions.

Walpole, Brenda. _Biology for the IB Diploma Second Edition._ United Kingdom: Cambridge University Press, 2014. Print.

For this reason the water used will be at room temperature (23° approx) and will be performed on the same day to allow no margin for any discrepancies.

A model cell was made of a visking tube filled with ‘cell contents’. The cell contents represent the cytoplasm of a cell structure membrane. The visking tube represents the plasma membrane of the cell. It acts as a semi-permeable barrier as

7.When water bath is ready, put each test tube into the water bath. Wait 5 minutes.