Egg Osmosis Lab Report

The purpose of this lab is to determine what is the concentration of the unknown solution along with what concentration would be more isotonic to the chicken egg?

Hypothesis: If the egg is placed into water, then it will increase in size since water is a hypotonic liquid because it contains more water than the egg.

Take the 5.15g of baking soda and pour it into the bottom half of the egg and securely put the top of the egg on.

Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.

Retrieve the solo cup with the egg in it and fill with the amount of vinegar that covers the egg appropriattly

The lid was then placed on the jar and an observation was made and recorded. Observations of the egg were then made after 12 hours and 24 hours had passed and the observations were recorded. After 24 hours had passed, the egg was removed from the syrup and rinsed with tap water and observations were made and recorded.

An egg has a semi-permeable membrane, thus processes like osmosis could occur. Osmosis is the movement of water molecules from an area of higher water concentration to an area of a lower water concentration. Osmosis is important, especially for living organisms, as they help distribute nutrients in the body. An egg’s mass would change when it is soaked in different substances. The goal of this experiment was to investigate how an egg changes through osmosis. This experiment was done to show how substances affect the mass of an egg. To start off, an egg’s mass was measured by putting the egg in a beaker then placing it on a mass scale. The beaker was then filled with vinegar and left alone for twenty-four hours. After a day, the egg was taken

Then place the other egg into a bowl and cover that egg with water. From this point, daily observation as well as photographing the eggs took place monitoring for any changes to the egg's shell. The fourth and final step was to drain the liquids, rinse each egg with water and observe for any visible changes. My independent variable was the type of liquid used, my controlled variable was the same time of day in the same amount of liquid, and my dependant variable was the time (days) in the liquid. Observing the results, I could see that the water egg remained the same as predicted.

Remove the egg, rinse and dry it. And determine it’s mass. Record. Place the egg in your beaker with enough of the 5% NaCl solution to cover it.

This is the final step of the experiment. The shell-less egg was now placed into a new cup, but very carefully, as the egg was more fragile than when it was soaked in vinegar. The egg in the new cup was then covered with water. The egg sat in the water for a 24 hour time period once again. During this 24 hour time period the eggs appearance and size were once again recorded. After the time period had completed, the egg was removed from the water and was very carefully patted dry. Since the egg was now swollen, extra care was needed when holding the egg. The swollen egg was placed on the scale on last time and its mass was recorded as M3.

Procedures: To start my experiment I am going to gather all my materials to conduct my experiment. listed under the materials section. I start by poking holes in the top of eggs and I add four different colors to represent the different heights. Red is for the height of 3 ft, blue is for the height of 5-feet, yellow is for the height of 7 feet, and green is for the height of 9 feet. Set up 8 ft ladder.

In conclusion, the data that was collected helped tell us that the solutions greatly affected the cell of the egg because it caused the egg’s mass to increase than decrease. Also saw how the solutions played a role in the phospholipid bilayer and saw how it altered the shape of the egg. However, it does not support my hypothesis on the affect the egg would undergo onced placed in sugar and water but sugar was not used in this experiment, but water was used and it didn’t affect the shape of the egg, all it did was cause the egg to be in a isotonic state. According to Khan Academy (2017), states that a cell is isotonic “When the extracellular fluid has the same osmolarity as the cell, there will be no net movement in or out of the cell”. Which

The eggs were each individually placed in one of the beakers and were taken out and weighed every 15 minutes for 60 minutes. Weighing the eggs helped to test the process of osmosis by finding out the mass before and after soaking in water, this will dictate whether water has moved in or out of the egg.

Cells in all living things have an outer layer known as the cell membrane. The structure of the cell membrane consists of the phospholipid bilayer organized by the arrangement of hydrophilic heads and hydrophobic tails. It is a selectively permeable membrane, where it divides the outer environment from the interior of the cell. It can control substances moving in and out of living cells. Certain molecules like gases, water, and food are permitted to pass the membrane through the method of diffusion. Diffusion refers to the process in which molecules move on the concentration gradient, where they move from an area of high concentration to an area of low concentration. A type of diffusion is known as osmosis. It is the diffusion of water moving across the selectively permeable membrane. In this lab, students will be using eggs to construct an experiment to get a better study on how osmosis works in a cell. The eggs will be soaked in vinegar solution to remove their shells to expose each inner layer that resembles a selectively permeable membrane. The egg shell is composed of calcium carbonate that would dissolves in acidic solution such as vinegar. In the chemical reaction, it releases carbon dioxide gas. After the removal of the egg shell, it will be ready to be able to construct the experiment.

4.14. The accuracy of the data is dependent on the correct controls being in place, Ensuring temperate stays the same throughout all solutions, making sure that the solutions are correct that the weight and size of the eggs are the measured correctly and that when the eggs are removed they are removed at the same time. Some of the eggs were not completely submerged in the solution this may cause some errors in the experiment and may have caused varying results. The eggs were of different sizes this also will cause the results to vary, Gathering eggs of exact size and weight would have ensured the results were correct. The eggs may not have been dried enough also causing results to vary; this can be fixed by ensuring that the eggs are thoroughly dried. The eggs were taken out at a time around about 24 hours, having an exact time to remove the eggs would have eliminated errors.