Electrophoresis Lab Report

Singee Nguyen Transformation and Electrophoresis Lab Report Purposes Discuss the principles of bacterial transformation. Describe how to prepare competent E. coli cells. Discuss the mechanisms of gene transfer using plasmid vectors. Discuss the transfer of antibiotic resistance genes and tell how to select positively for transformed cells that are antibiotic resistant. Discuss the mechanisms of action for restriction endonucleases. Discuss how a plasmid can be engineered to include a piece of foreign

Title - Gel Filtration and SDS Polyacrylamide Gel Electrophoresis (P.A.G.E) Gel Filtration, SDS-PAGE Objective – The purpose of this lab was to separate a mixture of Hemoglobin, BSA, Blue Dextran, and yellow food coloring by size into fractions using gel filtration. Also simple non-quantitative assay was performed to determine the fractions that contained proteins. Another objective was to analyze the protein content of being BSA or Hemoglobin and estimate the molecular weight of the proteins collected

In this experiment, we tested our hypothesis through the use of a PCR and gel electrophoresis. To begin this experiment, we gathered the materials needed. We gathered a screw cap tube that contained the instagene matrix, a 1.5 ml tube, and a small cup containing 0.9% saline solution, P-20 micropipette, P-1000 micropipette, pipet tips, PCR tubes, cap-less microtubes, Master Mix, and water baths. Next, I rinsed my mouth thoroughly for 30 seconds with the saline solution and spit it back into the cup

determination will be done using the Western blot technique. We will use the Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) method to separate proteins by their molecular weight. This method is often used to determine the purity and weight of a protein. Electrophoresis is a method of separating proteins based on their chemical and physical properties. Gel electrophoresis has a gel support mediums that includes polyacrylamide, starch and agarose. The gel is chemically inert, it is easy

DNA Gel Electrophoresis Summary In the lab, we were introduced to six different DNA samples, the Crime Scene and 5 suspects. Our Independent variable of the experiment was the restriction enzyme, and our dependent variable was the DNA sample that matched the Crime Scene DNA. With our variables stated. This splices up the DNA into segments that are ready to begin gel electrophoresis. Gel electrophoresis is a method of taking DNA samples and turning them into visuals that can be compared and analyzed

the process of electrophoresis. This process will allow the DNA to form bands that reflect certain characteristics. Electrophoresis is a very important process. This process uses an

PTC Taster Genomic Analysis Lab Report Laboratory Goals: 1. Determine Taster Phenotype 2. Isolate DNA from each individual 3. Determine Taster Genotype Hypothesis: If I am a taster, then my genotype for PTC taster must be either TT (homozygous dominant) or Tt (heterozygous) I – Results: This experiment aimed to investigate the allele frequency of the PTC taster gene (TAS2R38) in a small population, represented by the students in class. The genotype obtained from genomic

eggs and producing healthier chickens. Recently reports of severe gastroenteritis have been linked to eating raw or undercooked eggs. This has led to the FDA to look for possible sources of contamination. Scientists have now isolated bacteria from batches of eggs known to cause the illness, and they found that the bacteria are resistant to kanamycin. The contaminated eggs were found to have

Bacterial Transformation Lab Report Elizabeth Amanquah Genetics 311 Monday 2:00-4:50 Jan Trybula Abstract This study was undertaken to develop an understanding of bacterial transformation by plasmid DNA. This study enables one to observe various traits displayed by transformed bacterial cells. Four experiments were conducted that included (1) Bacterial Transformation, (2) Genomic/Plasmid extraction, (3) DNA Electrophoresis, and(4) Plasmid /Oxidation EMSA. In this study, a transformation

manner of death was natural. I determined this by using past labs and Anna's external autopsy examination. Through the 1.1.6 labs, I found that Alex's fingerprints were present along with Anna's hair, shoeprints, and Aspirin. Although Anna and Alex shared the same blood type, through restriction endonucleases and gel electrophoresis, I found that Anna's DNA was the exact same as the DNA found at the crime scene. Anna's external autopsy report stated that Anna had swelling in her abdomen and ankles, pre-mortem