Lab Report: 10 ELISA Purpose: This test is often used to see if you have been exposed to viruses or other infectious substances. It is frequently used to screen for present or past infections. Introduction: ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood. An ELISA or enzyme-linked immunosorbent assay is a method used in the laboratory to aid in the diagnosis of a wide range of diseases. This test is performed on blood or urine and is used for measuring the amount of a particular protein or substance in these bodily fluids, such as infectious agents, allergens, hormones or drugs. This test relies on the interaction …show more content…
Then you add 100ul of PBS to each well, and then remove it. After that, to each well using different tip each time add 100ul PBS to number 1, add 100ul B to number 2, add 100ul C to number 3 and add 100ml D to number 4. Incubate at 37C (Body temp) for 15 mins. After that, remove liquid using different tips. Next add 100ul PBS, and then remove using different tips. Add 100ul 2C AB to each well and incubate at 37C for 15 min. remove the liquid using different tips, add PBS then remove the PBS. Add 100ul of substrate to each well and incubate at 37C for 5 mins. A= HIV Antigens B= Positive control C= Donor 1 serum D= Donor 2 serum PBS= Phosphate Buffered Saline Results: A | B | C | D | 1 | 2 | 3 | 4 | - | + | - | + | Conclusion: For the first row, the results can back negative because that was the control. The second column was positive meaning for this control this is what a positive would look like. Now from the third column this donor serum is just negative for that antibody and the fourth row would be a positive for HIV antibodies. This test is medically important because it’s used for measuring certain hormone levels such as HCG in the pregnancy test, thyroid hormones, detecting dust and food allergies, detection of illicit drugs such as cocaine and methamphetamines. Also to measuring antibodies which are produced in auto-immune conditions such as Lupus and rheumatoid arthritis.
The ELISA test can also be used to detect antibodies that are produced in response to a specific antigen. Using information about how you completed this ELISA experiment, outline a procedure for testing for antibodies in the blood.
Known or suspected human acquired immunodeficiency virus (HIV) - positive patients, or severe diseases such as acquired immunodeficiency syndrome (AIDS) or tuberculosis.
Use to identify risks during pregnancy and so any problems can be identified and solved early
The Enzymes linked immunoabsorbant assay (ELISA) is a commonly used biochemical technique, often used in immunology as a way to detect the presence of an antibody or antigen in a sample.
30). According to Brunstein (2016), individual tests can be moved to a patient’s bedside or in other less formal settings to provide faster diagnosis, allowing for quicker response times when medical interventions are needed (p. 30). Bedside testing uses a method called point-of-care testing (POCT) and presents both new possibilities as well as challenges (Brunstein, 2016, p. 30). Brunstein (2016) points out some of the challenges are decreased sensitivity, specificity, or sometimes both, especially when compared to the testing that is done in the core lab (p.30). However, POCT can prove to be advantageous because of reduced response times for medical interventions. POCT with moderate sensitivity and high specificity have proven to be convenient, cost effective first-line screening tools (Brunstein, 2016, p. 30). Molecular POCT has positive implications for the future but there are some limitations. POCT used for molecular diagnosis (Madix) are limited to only
when this test is taken it can diagnose COPD even before you have any signs at all.
What is the reason for the test: It is a type of ventilator. A device that helps with breathing.
This test is used to test the amount of digoxin in the bloodstream. Digoxin is used to help treat congestive heart failure by increasing cardiac output, digoxin should remain at a therapeutic level in the bloodstream. It is important to check these levels frequently because a person could develop a digoxin toxicity. When levels are too low clients may experience shortness of breath, fatigue, and/or edema. High levels of digoxin could cause vomiting, diarrhea, dyspnea, an irregular heartbeat, blurred vision, and/or dizziness, the client should be assessed for both high & low level symptoms. Multiple medications, certain herbal remedies, electrolytes such as potassium & magnesium, & licorice candy can alter digoxin levels in the body. If I client
Your test results will be reported as values. You will compare your results to normal ranges that were established after testing a large group of people (reference ranges). Reference ranges may vary among different people, labs, and hospitals. For this test, reference ranges are:
Each of the 5 samples examined during the lab were able to be diagnosed with certain diseases pertaining to the symptoms they were found to display during the urinalysis.
Overall, ELISA is a good serological method in immunodiagnostics, especially for less developed communities due to its cost and efficiency. However, it does come with limitations, mainly due to the economic status of the geographical areas of infection and the infections the individuals are commonly exposed to. As well as the effect that multiple infections can have on the sensitivity and specificity of
Screening refers to testing individuals without a known condition. Screening is done to identify whether these particular individuals have the malady. In most cases, early detection of the disease means early treatment which results to better and superior treatment (Watson, Templeton & Russell et al, 2002). The selection and use of screening instruments should be determined by various factors. Validity is one of the factors that should be considered in the use of an instrument. This is the capability of the instrument used in the process to provide true and correct measurements. Reliability of the instrument should also be considered as it refers to the ability of the testing kit to measure and present consistent results for the same trial
Incubate all of the testing plate, inverted (agar on top), overnight at 37 degrees Celsius (98.6 degrees Fahrenheit). Incubate longer if necessary if at lower temperature.
Antigens are substances that provoke an immune response (they're the ultimate target for the immune system). Antibodies are simply proteins that are secreted as a result of the antigen provoked immune response. In short, antigens cause the disease and
important test of an individuals physical health since unhealthy levels can lead to heart attacks,