Emission-Excitation Matrices

630 Words Jan 26th, 2018 3 Pages
They constitute optical landscapes that extend over the dimensions of excitation and emission wavelengths {λex–λem}, and where fluorophores appear in the form of peaks. In the field of marine and freshwater biogeochemistry, EEMs have been used for the study of dissolved organic matter (DOM), being a comprehensive analytical technique with which to characterise a highly complex mixture of organic compounds (Hudson2006, Fellman2010, Nebbioso2013). Indeed, EEMs have served to advance scientific knowledge about the ecology and biogeochemistry of DOM in aquatic systems [1,2]. Most importantly, they have contributed to evidence that some fractions of DOM are highly reactive organic molecules that are involved in numerous ecosystem processes, such as bacterial uptake [3–5], metal binding [6,7][1,2][1,2], photoreactivity [8–10] and light attenuation [11]. Overall these findings suggest the major involvement of DOM in the global carbon cycle [12,13].

Despite the great potential for EEMs to increase knowledge about DOM behaviour in the environment, their interpretation and statistical treatment remain a challenge [14]. The spectral shapes of EEMs are complex mixtures of multiple and overlapping independent fluorescence phenomena, caused by the wide range of organic molecules contained in DOM. As only about 25% of…
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