Enzyme Activity Lab Report

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Enzyme Action: Testing Catalase Activity 6B
Ava Suda
Cor Jesu Academy

Enzyme Action: Testing Catalase Activity 6B
Introduction
Hydrogen Peroxide, or H2O2, is harmful to most living organisms but can be converted to oxygen and water before the damage is permanent. This is thanks to enzymes, the biological catalysts that increase the rate of reactions. Enzymes can be studied by measuring the rates of enzyme-catalyzed reactions. This can be done in a number of ways, including measuring the pressure of the product as it appears, measuring the rate of disappearance of the substrate, and measuring the rate of appearance of a product.
Purpose
To measure the rate of enzyme activity under different temperatures, enzyme
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Click the green “Collect” button to collect data, which will cease collection after 3 minutes. If the pressure becomes higher than 130 kPa, then the stopper will pop out. If this occurs, then loosen the plastic tubing around the stopper to relieve pressure. Once the data is collected, disconnect the tubing from the stopper, remove the stopper, and discard the contents in a waste beaker or sink. Move the cursor to where the values start increasing, and click. Drag the mouse pointer to the point where the pressure values stop increasing and release the button. Next, click the Linear Fit button to form a linear regression. Record the slope of the line in Table 4. The line regression box can now be closed. Repeat these steps with Test Tubes 2, 3, and 4, adding 2 drops to Test tube 2, 3 drops to Test Tube 3, and 4 drops to Test Tube 4, respectively.
To test the effect of temperature, place 4, clean test tubes in a test tube rack and label them “T 0-5,” “T 20-25,” “T 30-35,” and, “T 50-55.” Add 3 mL of 3% H2O2 and 3 mL of water to each test tube. To measure enzyme activity at 0-5°C, prepare a water bath with a temperature range of 0-5°C in a 600 mL beaker. Place Test Tube T 0-5 in the water bath and record the temperature in Table 4. Add 2 drops of the enzyme solution to the test tube, and connect the free end of the plastic tubing to the connector in the stopper. Click the “Collect” button to collect data, and

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