Enzyme Catalase Lab

The more acidic a substance is the less oxygen it will produce when going through a chemical reaction. During the Lab “How Do Changes in pH Levels Affect Enzymes Activity”, the researcher conducted an experiment to test the effects that an acidic, neutral, and a base substance will have when combine it with hydrogen peroxide. The data table shows that HCL (acidic substance) barley produced any oxygen at all when it was combining with Hydrogen Peroxide. The pH level for HCL was 2.5; this level indicates that the substance was very acidic. When the H2O and NaOH were tested they produced more bubbles than HCL. NaoH produced a little more bubbles than HCL. The pH that NaoH produced was a 9, which is a base. H2O produced more bubbles than both substances;

Enzymes are proteins in living organisms that speed up chemical reactions and are also vital in making sure our bodies can function. These chemical reactions are know as catalysts. However, enzymes can not create these reactions by themselves. So, they work with molecules called substrates to help them out. The enzyme and substrate would usually fit perfectly together, unless the enzyme loses its shape. Two major things that can impact the disfiguration of the enzyme's shape are changes in temperature or pH levels. In this lab, our goal was to show if enzyme activity differs due to changes in temperature and pH levels by using catalase solution (potato puree), hydrogen peroxide, Hydrochloric Acid solution, sodium hydroxide (base

The human body is an incredible system that is capable of working a multitude of diverse functions. Without the help of the many different protein molecules, the human body would not be able to function properly. One major group of proteins called enzymes are mandatory for essential life. These proteins are constantly at work assembling molecules, metabolizing energy, and fighting off infections. An enzyme is a macromolecule that acts as a catalyst that speeds up a chemical reaction without being consumed by the reaction. Without these proteins, these reactions would take place too slowly to keep us alive. Essential parts in your body like vitamins and minerals cannot do any work without

These results shown from this experiment led us to conclude that enzymes work best at certain pH rates. For this particular enzyme, pH 7 worked best. When compared to high levels of pH, the lower levels worked better. The wrong level of pH can denature enzymes; therefore finding the right level is essential. The independent variable was the amount of pH, and the dependent being the rate of oxygen. The results are reliable as they are reinforced by the fact that enzymes typically work best at neutral pH

Hold the IKI spray bottle 25 - 30 cm away from the paper towel, and mist with the IKI solution.

The purpose of this experiment was to record catalase enzyme activity with different temperatures and substrate concentrations. It was hypothesized that, until all active sites were bound, as the substrate concentration increased, the reaction rate would increase. The first experiment consisted of five different substrate concentrations, 0.8%, 0.4%, 0.2%, 0.1%, and 0% H2O2. The second experiment was completed using 0.8% substrate concentration and four different temperatures of enzymes ranging from cold to boiled. It was hypothesized that as the temperature increased, the reaction rate would increase. This would occur until the enzyme was denatured. The results from the two experiments show that the more substrate concentration,

The purpose of this investigation is to discover the effect of pH on the activity of catalase, an enzyme which plays the integral role of converting hydrogen peroxide into water and oxygen, and discover which pH level it will work at the most efficient rate (the optimum). The original hypothesis states that that the optimum would be at a pH is 7, due to the liver, where catalase usually resides, being neutral. The experiment consists of introducing the catalase to hydrogen peroxide, after exposure to certain solutions; hydrogen peroxide, water and hydrochloric acids, all containing the adjusted pH, and measuring the height of froth formed, an observable representation of the activity of the enzyme. The final data indicated that

After reviewing the basics of enzymes and catalysis, we take a dive into the wonderful

In this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes.

We used apple, potato, and chicken liver to prove that not only beef liver contains catalase. The group conducted three experiments: one contained potato and H2O2, another had apple and H2O2, and the last had chicken liver and H2O2. We added 2mL of hydrogen peroxide (H2O2) to all three test tubes. The bubbling effect proved that all three had catalase in them. We realized that the more the substance bubbled the more catalase it contained, and that the less it bubbled, the less catalase there was. We also rated the reactions by the speed of the reaction in seconds, like we learned in part

Background: Enzymes are proteins that increase the rate of reaction by lowering the activation energy. When an enzyme binds to a specific substrate, an enzyme substrate complex is formed, allowing for the reaction to take place over less time. Since the enzyme is recyclable, the reaction produces a product and the unaltered enzyme. Catalase is the enzyme used specifically for the decomposition of hydrogen peroxide. Furthermore, the amount of substrate used can be determined by measuring the amount of product formed in the reaction. If the amount of product formed over time is graphed, the graph will prove the rate of reaction to decrease over time as the availability for a substrate and enzyme to bind decreases. The initial velocity of an enzyme-catalyzed reaction is constant because the number of substrate molecules at the beginning of a reaction is typically vastly greater than the number of enzymes, causing the rate to be determined by the characteristics of the enzyme. When the substrate concentration decreases to a certain point, when enzymes and substrates do not easily collided in the specific manner to form the enzyme-substrate complex, the rate of reaction will decrease.

Enzyme catalysis is the increase in the rate of a chemical reaction by the active site of a protein. A catalyst is a substance that can help the reactants in a chemical reaction react with each other faster. The catalyst for this experiment is yeast. In this lab, the chemical combination of hydrogen peroxide and yeast are used to form a reaction of creating oxygen. The active sites of the yeast combines with the hydrogen peroxide and causes oxygen to form at various levels. Yeast is a one-celled organism belonging to the group of organisms called fungi. Yeast is sometimes used in genetic engineering to produce large quantities of enzymes that can be used for medical purposes as healing wounds and reducing inflammation (How Stuff

Organisms cannot depend solely on spontaneous reactions for the production of materials because they occur slowly and are not responsive to the organism's needs (Martineau, Dean, et al, Laboratory Manual, 43). In order to speed up the reaction process, cells use enzymes as biological catalysts. Enzymes are able to speed up the reaction through lowering activation energy. Additionally, enzymes facilitate reactions without being consumed (manual,43). Each enzyme acts on a specific molecule or set of molecules referred to as the enzyme's substrate and the results of this reaction are called products (manual 43). As a result, enzymes promote a reaction so that substrates are converted into products on a faster pace (manual 43). Most enzymes are proteins whose structure is determined by its sequence of its amino acids. Enzymes are designed to function the best under physiological conditions of PH and temperature. Any change of these variables that change the conformation of the enzyme will destroy or enhance enzyme activity(manual, 43).

This experiment is designed to analyze how the enzyme catalase activity is affected by the pH levels. The experiment has also been designed to outline all of the directions and the ways by which the observation can be made clearly and accurately. Yeast, will be used as the enzyme and hydrogen peroxide will be used as a substrate. This experiment will be used to determine the effects of the concentration of the hydrogen peroxide versus the rate of reaction of the enzyme catalase.

reaction rate increases. If the temperature of an enzyme gets to high the reaction rate will slow