Enzyme Catalysed Reaction Lab

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Experiment 3:

A change in concentration of an enzyme solution can greatly affect the action of an enzyme-catalysed reaction. A change in concentration will only affect the rate of reaction is it is a limiting factor and once the concentration has increased to the point that it can no longer affect the rate of reaction, then it no longer a limiting factor. This point is called the saturation point as seen in figure 6. (Alevelnotes, 2016)

This experiment supported the hypothesis that the stronger the concentration, the more efficient the enzyme catalysed chemical reactions. .As time continued, the amount of substrates that needed to be bind with an enzyme was reduced because most of the milk fats had been converted in to fatty acids, which then caused the rate of reaction to slow down as it was harder for the remaining substrates to collide with the enzyme. This means the moment where the reaction is the most effective is at the beginning, when the lipase solution was just added to the milk, which is known as the Initial Reaction Rate. This is reaction is displayed in graph 3, as all on the lines have a steep gradient at the begging of the test, which then begin to plateau towards the end of the reaction when the solution became colourless. It’s interesting that the 2% concentration had a higher initial reaction rate than the 4%
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This is the reason why the 0.25%, 0.5% and the 1% concentration never became colourless within 20 min unlike the 2% and 4% concentrations, which became colourless within 8 to ten minutes. This is due to an abundance of enzymes, which therefore increase the rate of reactions because it’s far easier for the enzymes to collide with the substrates. (Academic Brooklyn,
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