Enzyme Catalysis And Its Effect On The Reaction Rate Of An Enzyme

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PURPOSE: Measure the effects of changes in catalase concentration, substrate concentration, and salinity on the reaction rates of an enzyme.

RESEARCH COMPONENTS: What is being tested-what do you know about enzymes?

Explain how the structure of an enzyme is related to its function/activity.
All enzymes are structured to react with unique substrates. and each enzyme has an active site where the substrate bonds to the enzyme. The active site of an enzyme is shaped to fit the specific substrate it reacts with.

Explain how free energy (G) relates to activation energy and enzymes.
Free energy (G) is the amount of energy required to do work. Enzymes help speed up the process by lowering the activation energy needed to complete a reaction.

Explain how temperature, pH, enzyme concentration and substrate concentration would affect reaction rates.
Temperature affects the rate of which the enzyme reacts, enzymes react typically faster in hotter temperatures than colder. Ph levels that are extremely low or high can stop enzyme activity completely or slow them down.If there is more enzyme concentration the reaction is quicker because enzymes become constant. Also if substrate activity increases, so the reaction time speeds up until maximum is reached.

For each part of the lab (I, II, III) identify the control (if any), the independent variable, and the dependent variable.

Part I
Part II
Part III
type of enzyme,
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