Enzyme Catalysis Lab

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Abstract: After reviewing the basics of enzymes and catalysis, we take a dive into the wonderful world of catalase. Beginning with establishing a base line of just how much hydrogen peroxide there is in 5.0mL of the reacted solution; to figuring out exactly how much actually reacted after 300 seconds of catalyzed reaction. Follow the experiment from the beginning steps right to the end as you see where the students went wrong, interpretation of the results, and great answers to work sheet questions. Now, dive into the amazing world of catalase catalysis. Background Information: Enzymes are catalytic proteins, meaning they speed up – but do not create – chemical reactions, without being used up or altered permanently in the…show more content…
Each enzyme has specific ranges at which it optimally functions; in general, increasing the temperature will help the reaction along, until the point at which the protein degrades and denatures – or falls apart into its lower levelstructures. Denatured proteins will often return to their original state, after the removal of the denaturing agent, except when they are degraded multiple levels (such as Quaternary to Secondary). Rate of reaction through catalysis can also be increased by increasing the concentration of either the enzyme or the reactants; enzyme if all the active sites are full or the substrate if the active sites are not all full. I predict that the reaction will begin rather quickly with the addition of the enzyme, but will slow as the H2O2 is converted to H2O and O2. I also believe that, given the amount of time the reactions are to be run for, not all of the H2O2 will react, thus leaving some to react with the KMnO4 even after the full 360 seconds. Materials and Methods: For the Enzyme Catalysis Lab, each of eight groups was assigned to perform a baseline measurement and a catalyzed reaction for the time period of their choice (10, 30, 60, 120, 180, 240, 300, and 360 seconds). My group chose to perform the 300 second (five minute) timeframe. We began the lab by gathering the glassware, the plastic ware, the hydrogen
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