Enzyme Concentration And Incubation Time

with phosphate saline buffer. The enzymes(trypsin and collagenase) were dissolved in Dulbecco’s Modified Eagle’s Medium (DMEM) (Hi Media) supplemented with 50µg/ml gentamycin (Hi Media),10µg/ml Amphotericin (Hi Media) and 50µg/ml of glutamine(Hi Media). Tissue was subjected to sequential enzymatic digestion. The first digestion was done with trypsin in shaking

LABORATORY REPORT Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature, amount

Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation time Effect

no bone loss. The enzyme, alkaline phosphatase(AP), is proved to slow down the effects of hypophosphatemia. Few individuals with hypophosphatemia and no bone loss have found a mutated version of AP in their blood. To observe the behavior and functionality of the mutant AP enzyme versus the Normal AP enzyme, we test and compare them in different pH levels as well as different concentration levels. The first step in our experiment was determining the wave length in which the enzyme functioned the best

on the panel above to save your report) Activity: Name: Instructor: Date: Enzyme Activity Pam Campbell Id 0002337 Dr. Murphy Nmezi August 9, 2011 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced

Determination of the activation energy of an enzyme catalysed reaction Introduction In this practical the aim for this experiment was to find out the catalytic power of alkaline phosphate, as well as the rate of reaction and the activation energy of p-nitrophenol phosphate. Enzymes are biological molecules that catalyse a chemical reaction. ‘Enzymes work by lowering the activation energy of a chemical reaction making it easier to proceed’ [1]. This allows molecules to have more energy therefore

INTRODUCTION α-Amylases(E.C. 3.2.1.1.) are starch-degrading enzymes that catalyze the hydrolysis of internal a-1,4-O- -glycosidic bonds in polysaccharides with the retention of a-anomeric configuration in the products. Bacillus amyloliquefaciens find potential application in a number of industrial processes such as in food, fermentation, textiles and paper industries [1]. Most of the a-amylases are metalloenzymes, which require calcium ions (Ca2+) for their activity, structural integrity and stability[2]

– to weigh out mass of enzyme needed * Exposed, developed photographic film – as substrate * 4g Encapsulated Alcalase – as high temperature protease enzyme * 4g Encapsulated Savinase – as low temperature protease enzyme * Water bath – to incubate boiling tubes holding photographic film at temperatures 30°C -100°C at 10°C intervals * 400cm3 pH8.0 buffer – to maintain

Green 4 (80% in 24 h), Diamond Green 4 (complete decolorization in 24 h), Pigment Orange 31 (complete decolorization in 3 h) and RO16 (complete decolorization in 2 h), while in sterile, cell free medium decolorization did not occur up to 48 h of incubation suggesting the absence of abiotic decolorization. As RO16 showed complete decolorization in 2 h, this industrially important toxic azo dye was taken for further studies. Lysinibacillus sp. RGS could decolorize RO16 rapidly and thus was found to

of different drugs on various parasitic organisms we are able to distinguish the type of disease or infection that is present as well as the mechanism of action that takes place by each drug in question. The drugs may function by interacting with enzymes such as transpeptidase and thymidylate synthetase, for example Penicillin and 5-FU function respectively. The effectiveness can be quantified by measuring the zones of inhibition created by the drug on the plate of the bacteria or fungus. This is