Enzyme Concentration Lab Report

At five minute intervals over the next fifteen minute period, record the color intensity of the solution of each test tube.

The same type of experimental process was used in the other experiments of temperature, and enzyme, substrate, and ionic concentration. For example, for temperature, they replaced the distilled water in the cuvettes with water of different temperatures of 3, 15, 25,37,and 100 degrees Celsius. Then, after the colorimeter was calibrated, the absorbance and transmittance of the data was collected for 2 minutes in increments of 20

There were a couple of problems that our group encountered while conducting this experiment. Since this was our first experiment dealing with enzyme activity, the probability of human error increased. We found it difficult to go through the procedures with undefined roles for our team. The other problem that our team encountered was the getting the cuvettes into the spectrophotometer quickly once the enzyme was introduced to the cuvette.

In the initial part of the experiment, the materials used were: cuvettes, broad-range pH paper, dry watch glass, a spectrophotometer, parafilm, transfer pipette, solution E (solution B and distilled water), sodium carbonate, hydrochloric acid, and solution D (enzyme ALP high concentration). The experiment was initiated by preparing solution E. Solution E was formed by adding 6.5 mL of distilled water (dH20) with 6.5 mL of solution B (para nitro phenolphosphate - pNPP). A total of four cuvettes were labeled control 1, acidic 2, neutral 3, and 4 basic (Wilson, et al 2015). Each cuvette contains a specific pH with the exception of the control cuvette as shown in Table

The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically, the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase.

Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology Department, 2011).

The motive of this lab is to attain a better understanding of enzyme activity by timing chemical reactions in certain temperatures and pH levels. Enzymes act as catalysts that help speed up reactions. Without these enzymes chemical reactions in metabolism would be backed up. There are two factors that affect an enzyme’s reaction rate: temperature and pH levels. In this label we will be testing different pH levels and temperatures to see which ones cause the most reactions.

Once each test tube had been in the respective temperatures for a minimum of 10 minutes, test tube 1 was taken out of its bath and the rest of the tubes were left in their respective places. To find the rate of enzyme activity for test tube 1, 10 drops of enzyme solution were added, and the timer began. The contents were poured into a clean 250mL Naglene bottle, and swirled to mix. Next, the O2 Gas Sensor

The purpose of this lab is to test for enzyme activity by examining factors that may influence enzymes.

Controlled Variables: amount of substrate/enzyme present, pH, overall amount of solution within each test tube, and temperature.

Enzymes are proteins that catalyze (speed up) biological reactions in an organism by lowering the activation energy of a reaction. They do this by either straining the bonds in a molecule so that is easier to break up or by placing separate molecules/elements close to each other so that bonds are formed. Enzyme activity is influenced by an array of different factors such as enzyme concentration, substrate concentration, temperature, pH and inhibitor concentration. All of these affect the rate of reactions of enzymes and some such as temperature, inhibitors and pH can under circumstances cause enzymes to become permanently affected. Catalase is an enzyme found in almost all organisms on earth exposed to Oxygen.

The Effect of low pH on Enzyme Activity Frank Keith Welsh, BIO 102, Fall semester Today I will be providing an experiment on the effects of pH on enzymes. Enzymes are affected by changes in pH. Exceptionally high or low pH values commonly cause in complete loss of activity for most enzymes. Furthermore to include temperature and pH there are other elements, such as ionic strength, that can shake the enzymatic reaction. To each of these both physical and chemical parameters should be considered and optimized for an enzymatic reaction to be precise.

To study the effects of temperature, pH, enzyme concentration, and substrate concentration there were certain steps that were followed in order to conduct this experiment. Each factor had a separate procedure to follow to find how each had a different effect on the enzyme.

In this lab or experiment, the aim was to determine the following factors of enzymes: (1) the effects of enzymes concentration the catalytic rate or the rate of the reaction, (2) the effects of pH on a particular enzyme, an enzyme known and referred throughout this experiment as ALP (alkaline phosphate enzyme) and lastly (3) the effects of various temperatures on the reaction or catalytic rate. Throughout the experiment 8 separate cuvettes and tubes are mixed with various solutions (labeled as tables 1,3 & 4 in the apparatus/materials sections of the lab) and tested for the effects of the factors mentioned above (concentration, pH and temperature). The tubes labeled 1-4 are tested for pH with pH paper and by spectrophotometer, cuvettes 1a-4a was tested for concentration and cuvettes labeled 1b-4b was tested for temperature in four different atmospheric conditions (4ºC, 23ºC, 32ºC and 60ºC) to see how the enzyme solution was affected by the various conditions. After carrying out the procedures the results showed that the experiment followed the theory for the most part, which is that all the factors work best at its optimum level. So, the optimum pH that the enzymes reacted at was a pH of 7 (neutral), the optimum temperature that the reactions occurs with the enzymes is a temperature of 4ºC or

To prevent fluctuation in the pH, a solution known as a “buffer solution” was used in the experiment. Buffer solutions are mixtures of at least two chemicals which counteract the effect of acids and alkalis. Therefore, when a small quantity of alkali or acid solution is added the pH of the enzyme doesn’t change.