Enzyme Functions And How The Temperature Of The Reaction Mixture Influences Starch Synthesis

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Unit 4 - Starch Synthesis
In this lab, I tested in which conditions starch synthesis will take place and how the temperature of the reaction mixture influences starch synthesis. My main goal for this experiment is to learn about the basic principles of enzyme functions, and how their environment around them can influence them.

Hypotheses:
I hypothesized that glucose would give off the most starch, compared to the other solutions, and I also predict that the room temperature test tubes would be the most efficient at synthesizing the starch. My null hypothesis was that none of the solutions would have an effect on the starch synthesis. Also, the temperature of the solution would not have an effect on the starch synthesis. Both of these
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We placed the 2 plastic centrifuges in the centrifuge, which spun the solution for 3 minutes at 6000 rotations per minute (rpm). We then poured the upper layer liquid (supernatant) from both the plastic centrifuges into a clean beaker, leaving the white pellet in the plastic centrifuges. Then, we removed a small amount of the solution from the beaker, and placed it in the spot plate, adding a drop of iodine. By adding the iodine, it would tell me if the starch was present or not. Given that the solution did not turn blue or black, we can say that the solution tested negative, indicating starch was not present. We then put the test tube E’s in their perspective hot water bath, one in each of the following; 40℃, 60℃, and 80℃ for about 10 minutes. After taking out all of our test tube E’s, we added 30 drops of starch-free enzyme extract (the solution that we just tested), from the beaker to all of the test tubes (all 8). We mixed all of the solutions by hand, where we then added a small drop of soluble starch to each test tube, that we also mixed by hand. Next, to test the solutions/contents, to see if starch was present, set up my spot plate so that it would be organized to conduct the experiment. We did this by taking tape and labeling the left side of the spot plate, with letters of the test tubes that would be tested. Above the spot plate we laid a piece of tape along the top of the spot plate, labeling with the amount of time that

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