Enzyme Lab

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Enzyme Activity Lab


1] Are enzymes reusable? Yes, enzymes are reusable.

2] Which of the following do you think contain catalase? [Manganese dioxide, chicken meat, apple, carrot, potato, or chicken liver] I think that the manganese dioxide, apple, and carrot contain the catalase.

3] Would grinding the materials affect enzyme activity?

I think that grinding the materials will increase enzyme activity.

4] Would temperature affect enzyme activity?

I think that at normal body temperature, regular enzyme activity will occur.

5] What about pH levels?

I think that at a balanced pH level (pH 7) like water, enzyme activity will be at the highest.


-To demonstrate the activity of
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The tissue with the highest average rate of reaction was liver. Liver had an average rate of reaction of 3. Because the body creates so many deadly poisons, catalase needs to be present in order to speed up the rate of reaction. The liver and the hydrogen peroxide or H2O2 reacted through hydrolysis. The hydrogen peroxide or H2O2 was separated by taking out the water that bonded it. The gas that was produced was O2 or oxygen. One can tell that O2 or oxygen was produced because it the only gas the supported burning. When the class did the demo, the splint stayed lit in the test tube, proving that oxygen was present. The liquid that was left behind was H2O better known as water. When hydrolysis took out the oxygen in the bond, the only thing that was left was H2O2.

The group data compared to the class averages weren’t that different. To start with, the used hydrogen peroxide or H2O2 with the new liver were off. The rate of reaction that the group gave was a 1 while the class average was .25. This might have happened by not pouring all of the used hydrogen peroxide or H2O2 into the new test tube with the new liver. Also, the warm liver and hydrogen peroxide or H2O2 varied by a 2.33 difference. This may have occurred by not having a constant temperature of 25°C. Another big difference that we had was with the hydrogen peroxide/ H2O2, liver, and acid pH3. Our group data showed that we had given the rate of reaction a 3

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