Enzyme Lab Report

If temperature of the water(enzyme environment) is increased to 35°C, then the enzyme activity will

• Fourthly, we kept the temperature at a constant 25°C using a water bath. At low temperatures, an increase in temperature causes an exponential increase in enzyme activity. This is because an increase in temperature provides more kinetic energy for the collisions of enzymes and substrates, so

These results show how temperature of extreme high, or low affects enzyme activity. The highest rate of enzyme activity occurred at 37 Cº. Anything that was hotter or cold than 37 Cº slowed the reaction rate. As I thought, 100 degrees would denature the enzyme, and that was the case. The data provided shows exactly what temperatures enzymes work best, and worst. The objective was achieved as we discovered the different reaction rates under different temperatures. The results are reliable, as we know enzymes do not work well when under extreme heat or denaturation occurs. What I learned in this experiment was that enzymes don’t work well under cold temperatures because they tend to move slower. My hypothesis did not quite match, because I thought they work best at lower temperatures.

The graph below shows the effect of temperature on relative rate of action of enzyme X on a protein.

All enzymes have an optimal temperature where activity is the most productive. If the temperature of the enzymes environment is too low or too high, the enzyme and the substrate will not interact in a desired way which results in decreased activity. High temperatures of enzymes lead to high energy bonds breaking, thus altering the active site and overall enzyme by denaturation. If an enzyme temperature is too low the lack of activity may be due to lack of energy for substrate to interact with enzyme. By this knowledge it is expected that a temperature-rate plot of an enzyme should result in a bell curve with the optimal temperature being the highest point.

enzyme reaches a low temperature the reaction rate slows, as an enzymes temperature rises the

As the temperature increases, so will the rate of enzyme reaction. However, as the temperature exceeds the optimum the rate of reaction will decrease.

· I predict that the enzyme will work at its best at 37c because that

Do Different Temperatures of Hydrogen Peroxide Affect Enzyme Activity? Purpose To test the activity of an enzyme called catalase when hydrogen peroxide is at different temperatures. Background Hydrogen peroxide (H202) is a chemical that is a by-product of many chemical reactions in living cells. Hydrogen peroxide is also extremely toxic to human cells.

Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC, which is the body temperature, where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the enzyme will stop working.

To find the effect of temperature on the activity of an enzyme, the experiment deals with the steps as follows. First, 3 mL if pH 7 phosphate buffer was used to fill three different test tubes that were labeled 10, 24, and 50. These three test tubes were set in three different temperature settings. The first test tube was placed in an ice-water bath for ten minutes until it reached a temperature of 2° C or less. The second tube’s temperature setting was at room temperature until a temperature of 21°C was reached. The third tube was placed in a beaker of warm-water until the contents of the beaker reached a temperature setting of 60° C. There were four more test tubes that were included in the procedure. Two of the test tubes contained potato juice were one was put in ice and the other was placed in warm-water. The other two test tubes contained catechol. One test tube was put in ice and the other in warm water. After

The results in Figure 1, supported both the hypothesis as well as the predictions because the graph depicts how the differences in temperature changed the enzyme activity of amylase. The results concluded that as temperature deviates from optimal temperature amylase will hydrolyze starch at a lower rate. At 5 and 70 degrees Celsius amylase took longer to hydrolyze starch compared to 25 and 40 degrees Celsius. The results also supported the prediction that at 40 degrees Celsius, enzymes will break down starch at a faster rate than at 5 degrees Celsius, 25 degrees Celsius, or 70 degrees Celsius. The results showed 40 degrees Celsius as the optimum temperature for the hydrolysis of starch because 40 degrees Celsius is close to the body’s temperature.

The sides of the beaker may also still be hot. If this method is not used when transferring chemicals, it may result in injuries such as burns and cuts from the glass of the beaker or test tube. The last lab technique learned in the lab is using a piece of aluminum wire to dislodge any copper precipitate forming on the

The effect of temperature and ph level of an enzyme activity was then tested. When temperature increases, the kinetic energy, energy at work, increases. At first, the increase of temperature will increase the rate of reaction because enzyme catalase reacts by colliding with substrate molecules. (Ottaway, J. H). The

Biology 121 KS Lab Report IV 03/06/2018 Jannet Marin Testing the Effects of Temperature and pH on Potato Catalase Activity Introduction: To bear life in the cell, nearly all metabolic processes need enzymes. Enzymes are “macromolecular biological catalysts that help to speed up, or catalyze, chemical reactions.” The activity of enzymes is also affected by changes in the pH and temperatures. However, tremendously high or low pH and temperature values could cause complete loss of activity for most enzymes. Catalase is the most common enzyme found in potatoes and almost all organisms that are exposed to oxygen.