Enzyme Properties Under Altered Conditions

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Enzyme Properties under Altered Conditions

Melissa Piccione
BIOL 1500
Instructor: Dr. Christopher Blanar
Date: 7 October 2015

Abstract: An important part of the function of enzymes is dependent on their structure. Factors that affect this functionality are temperature, concentration, and pH. The effects of pH, enzyme concentration, and temperature on the catalytic rate of various enzymes were explored through three controlled laboratory experiments on alkaline phosphatase’s (ALP) ability and rate at which it converts the substrate dNPP to PNP. The results indicated that the enzyme is most effective in a neutral environment and that its reaction rate is directly proportional to both concentration and temperature. The
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Things like kinetics, type, and intensive properties are all possible causes that can effect change on enzyme behavior. Specifically, the effect of pH, temperature, and enzyme concentration of alkaline phosphatase (ALP) was examined in this experiment. (Wilson, 2015)
It is often the case that an enzyme is almost entirely effective only under a specific pH range. Once beyond the range, the enzyme’s behavior exhibits almost no productive activity. The pH where the enzyme is most effective is referred to as the optimal pH. Most enzymes within human functions and cells have optimal pHs around a neutral pH, though certain enzymes exhibit prime functionability under extremely acidic or basic pHs.
Like optimum pH, enzymes also have an optimum temperature during which they are most effective. Most enzymes experience this at 20° C and 40° C, though enzymes located in colder or hotter environments are subsequently more efficient under more extreme temperatures. Most enzymes abide by a general double in reaction rate for every 10° C increase in temperature. This relationship does not hold, however, after a certain temperature because increased temperatures denature the enzyme and it then cannot catalyze. (Wilson, 2015)
Both the concentration of the enzyme and the concentration of the substrate control the rate of an enzyme controlled reaction. The rate of reaction increases with increasing concentration; however, if either substrate or enzyme becomes limited the reaction
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