Enzyme Properties Under Altered Conditions

1532 Words Oct 31st, 2015 7 Pages
Enzyme Properties under Altered Conditions

Melissa Piccione
N01696299
BIOL 1500
Instructor: Dr. Christopher Blanar
Date: 7 October 2015

Abstract: An important part of the function of enzymes is dependent on their structure. Factors that affect this functionality are temperature, concentration, and pH. The effects of pH, enzyme concentration, and temperature on the catalytic rate of various enzymes were explored through three controlled laboratory experiments on alkaline phosphatase’s (ALP) ability and rate at which it converts the substrate dNPP to PNP. The results indicated that the enzyme is most effective in a neutral environment and that its reaction rate is directly proportional to both concentration and temperature. The latter of those two may be accounted for in procedural errors, as the results do not coincide with accepted enzyme behavioral ideals.
Introduction:
Enzymes, by definition, are proteins that speed up chemical reactions. The enzymes act as catalysts for every chemical reaction occurring within cells. Enzymes also act as digestive agents, breaking down food to access the energy and nutrients carried within. Additionally, enzymes are instrumental in testing and diagnosing many types of diseases and as such are invaluable to the medical field. Without enzymes involvement in the biological processes, life itself would cease to carry on.
Unlike inorganic catalysts, enzyme activity can be altered or controlled by different properties, both external…
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