Pre-Lab Questions 1. How could you test to see if an enzyme was completely depleted during an experiment? My understanding is that enzymes have the capacity of acceleration a biochemical reaction without being consumed or depleted (Santhosh, 2017). However, a possible way to detect if the enzyme is still reacting is by seeing if the reaction is still occurring. For instance, in experiment two, after about a minute, the reaction stopped bubbling and producing gases. 2. Describe an experiment that
and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm, a wavelength which is best for recording the absorbance values for the experiment. From the results, 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also, 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the
Unit 1 Lesson 1 Key Question Biology SBI4U-C ILC 1) Mg2+ Has 10 electrons. 2) Carbon -14 would not be effective at dating bones that are millions of years old. Carbon -14 is effective at dating to a maximum of 40 000 to 50 000 years old. The isotope decays over time and would not be present on bones that are millions of years old. 3) Hydrolysis - Water used to breakdown molecules. Hydro means water and lysis means to breakdown. An example of hydrolysis in action within our bodies is how our
Question # 1: How does the concentration of substrate affect the reaction rate? Hypothesis: If the concentration of a substrate increases, any time an enzyme happens upon a substrate it will be free to help that substrate react, but then when an enzyme encounters a new substrate at a slightly higher concentration it will already be working on another substrate, therefore, as we increase substrate concentration it is not going to increase as fast as the reaction rate. Argument: Substrates are
The Effect of low pH on Enzyme Activity Name Institution Affiliation Outline Enzyme: Amylase Source: Barley Substrates: Starch from potatoes Iodine Method: Time-based experiment on the enzyme 's reaction to converting Starch into sugars by testing the amount of time in which a 2 cm3 of starch solution would be converted to sugars. Control Group: • Amount of Iodine • Amount of Starch Sample Size: 1.50 g Introduction Enzymes are typically proteins and act as catalysts for various
following questions: How did the rate of the reaction change as you changed each of the three variables? If you vary the temperature, after a certain temperature, the enzymes will become denatured and then the rate will decrease. Each enzyme functions at an optimum pH. Well according to the graph, you see that the enzyme did not function at all when boiled which can be explained by the fact that it got denatured. Denaturing means the secondary and tertiary structure of the enzyme
Decomposition of Hydrogen Peroxide with the Enzyme Catalase Purpose Question and Background Information: What are the effects of different temperatures on the decomposition of Hydrogen Peroxide with the Enzyme Catalase. Different enzymes work best at different optimal temperatures, and with this experiment we hope to discover which temperatures are unideal for Catalase. The substrate is H2O2 which binds to the active site of the enzyme Catalase. The reaction in question is as written below: Hydrogen Peroxide
Enzyme Action: Testing Catalase Activity 6B Ava Suda Cor Jesu Academy Enzyme Action: Testing Catalase Activity 6B Introduction Hydrogen Peroxide, or H2O2, is harmful to most living organisms but can be converted to oxygen and water before the damage is permanent. This is thanks to enzymes, the biological catalysts that increase the rate of reactions. Enzymes can be studied by measuring the rates of enzyme-catalyzed reactions. This can be done in a number of ways, including measuring the
fats occurs. I will define Key Terms that describe what will occur in the experiments; I will conduct an experiment for each Activity and provide all resulting Data as well as answer Questions from each Activity. I will then provide a short Summary for what I learned in each Activity. Key Terms: Enzymes (p. 105) – Speed up chemical reactions in living things. Biological catalysts. Catalysts (p. 105) - is any substance which makes a chemical reaction go faster, without itself being
Activity Produced by Enzymes Christopher Gonzalez-Riano Panther ID: 5439112 Lab Partners: Mariana Loys, Marc Pugliese, Alexis Delgado Abstract: All biological processes require a constant supply of energy. This energy is produced through multiple chemical reactions that occur in cells and are controlled by biological catalysts called enzymes. Enzymes increase the rate of reactions by reducing the activation energy required to start a reaction. What effects an enzyme whether it is in a