Essay Enzyme Substrate Complex

1491 Words Jul 4th, 2013 6 Pages
Meera Patel
Enzyme lab
6/3/2013

Assignment 1 :- Effect of Temperature on Invertase Activity, Determining the Starting Velocity.
Enzyme is the fastest known catalyst. They increase the velocity and rate of reactions in living cells. Diverse range of proteins works as enzyme with their specific temperature point, pH etc. Enzymes react with the molecules known as substrates. Binding site of an enzyme and a substrate is known as active site. The amount of enzyme present in a reaction is measured by the activity it catalyzes. The relationship between activity and concentration is affected by many factors such as temperature, pH, etc. Though enzymes are important for biological reactions, certain factors play an important role in the
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Once, the enzymatic reaction attains its maximum point, there would be no increase in the rate of the reaction. The optimum value of the inveratse remains same under any conditions. Enzymatic reaction will not take place if it cannot reach its specific optimum values whether it is pH, temperature, substrate concentration or any other factor.
Assignment 2:- pH optimum for Invertase
Hypothesis: Differences in the pH will affect the rate of the velocity of the enzyme activity.
What is the optimal pH for invertase activity? Why and how do pH changes affect invertase activity?
Different enzymes have different pH optimal value that shows at which pH value it can work at its best. The optimum pH of the Invertase is around 4.0, which means that at this pH invertase functions properly and maintains the 3D structure and shape. At lower than 4 and higher 4.0 pH, inveratse gets denatured and its shape is ruptured resulting in the loss of its ability to function properly. From the graph, we can see that it is not active at the pH lower than 2.0.

Series | Best pH | 1 | 4.0 |

If the slope of the line to the left of maximum velocity is different from the slope of the line to the right of maximum velocity, explain why this is. What is responsible for these differences in enzyme kinetics?

There is an difference between the slope of line on right and left of the optimum
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