Enzymes Essay

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Introduction: Enzymes are an important part of all metabolic reactions in the body. They are catalytic proteins, able to increase the rate of a reaction, without being consumed in the process of doing so (Campbell 96). This allows the enzyme to be used again in another reaction. Enzymes speed up reactions by lowering the activation energy, the energy needed to break the chemical bonds between reactants allowing them to combine with other substances and form products (Campbell 100). In this experiment the enzyme used was acid phosphates (ACP), and the substrate was p-nitrophenyl phosphate. Enzymes are very specific in nature, which helps them in reactions. When an enzyme recognizes its specific substrate, the…show more content…
In this experiment, NaOH was the inhibitor used to stop the enzymatic reactions. NaOH is very basic and when added to a solution, will cause a drastic increase in pH, causing denaturation of the enzyme. The amount of product formed could be calculated by placing the test tube in a spectrometer after the addition on NaOH. A spectrometer measures the absorbance of a solution, which helps compare how much of a substance is in a solution. I hypothesized that the rate of the reaction would increase, producing more product as the amount of ACP in solution was increased because more enzymes allow for more substrate to be converted to product. The same hypothesis was made that when we increased the substrate, p-nitrophenyl phosphate, the amount of product produced would increase as well because there would be more substrate that could bind to the enzyme and be converted to product. For the environmental experiments, both temperature and pH, I predicted that the amount of product formed would increase with the temperature and pH, but then begin to decline after the enzymes reached optimal conditions. In other words, at the optimal temperature and pH, the enzyme velocity would be greatest, producing the most p-nitrophenol. Also, I predicted when the pH and temperature
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