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Enzymes Lab Report

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How does the concentration of the enzyme in different tissue types affect the efficiency of Catalase in its efforts to reach a dynamic equilibrium within the cell?

Enzymes undergo processes in order to maintain dynamic equilibrium at all

organisational levels. Enzymes are biological catalysts that help complex reactions

occur (Chem4Kids, 2015). A catalyst is a substance that increases the rate of a

chemical reaction. This is justified because enzymes accelerate the rate in which a

reaction occurs through the lowering of the reaction’s activation energy. Without

enzymes, the reactions in and between cells would be too slow to support life and

therefore the organism would die.

According to ‘Wiley Online Library’ (n.d), enzymes …show more content…

It requires no energy.

Once in the cytoplasm the RNA’s nucleotides are translated into Amino Acids

(Three nucleotides= one Amino Acid).

Once fully formed, the Amino acids are covalently bonded to one another to

produce a protein. Covalent Bonding is a chemical bond that involves the

sharing of electron pairs between atoms.

The Protein chain then either is developed in the Rough Endoplasmic

Reticulum and/ or moves straight to the Golgi body for transportation within or

out of the cell depending on its function.

All known enzymes comprise of proteins consisting of a long string of amino acids.

The arrangement of the amino acids determines the enzymes function. This is

justified through the investigation of the enzyme’s active site. As highlighted by

‘Kscience’ (2014), an active site is a specially shaped area of the enzyme that

connects with the substrate allowing a chemical reaction to take place (See Image
One, below). In the synthesis of enzymes the shape of the active site differs and therefore the substrate in which it reacts with will also change. Enzymes are very specific and work to complete one task.

How Enzymes …show more content…

This links to the concentration differences between plant and animal cells. Catalase has a higher concentration within animal cells because the cell functions of an animal cell produce more hydrogen peroxide as a by-product and therefore more Catalase is required to convert the hydrogen peroxide.

The results recorded from trial three deviated from the trend shown in the first two trials. Reasons for this abnormality within the data could be explained by the fact that the tissues spent over 48 hours in the fridge. As a result, the particles have a tighter bond and therefore the energy level of the catalase differs. Overall in the animal cells, there were more bubbles produced in the third trial than either the first or second trial. This is because once taken out of the fridge and added to the hydrogen peroxide solution, an exothermic reaction occurs (heat released). This is justified because the bottom of the test tube was hot to touch. As highlighted by… as the temperature increases, the rate of the enzyme’s reaction speeds up. This links to the experimental results, the bubbles released from the third trial increased due to the
amplified

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