How does the concentration of the enzyme in different tissue types affect the efficiency of Catalase in its efforts to reach a dynamic equilibrium within the cell?
Enzymes undergo processes in order to maintain dynamic equilibrium at all
organisational levels. Enzymes are biological catalysts that help complex reactions
occur (Chem4Kids, 2015). A catalyst is a substance that increases the rate of a
chemical reaction. This is justified because enzymes accelerate the rate in which a
reaction occurs through the lowering of the reaction’s activation energy. Without
enzymes, the reactions in and between cells would be too slow to support life and
therefore the organism would die.
According to ‘Wiley Online Library’ (n.d), enzymes
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It requires no energy.
Once in the cytoplasm the RNA’s nucleotides are translated into Amino Acids
(Three nucleotides= one Amino Acid).
Once fully formed, the Amino acids are covalently bonded to one another to
produce a protein. Covalent Bonding is a chemical bond that involves the
sharing of electron pairs between atoms.
The Protein chain then either is developed in the Rough Endoplasmic
Reticulum and/ or moves straight to the Golgi body for transportation within or
out of the cell depending on its function.
All known enzymes comprise of proteins consisting of a long string of amino acids.
The arrangement of the amino acids determines the enzymes function. This is
justified through the investigation of the enzyme’s active site. As highlighted by
‘Kscience’ (2014), an active site is a specially shaped area of the enzyme that
connects with the substrate allowing a chemical reaction to take place (See Image
One, below). In the synthesis of enzymes the shape of the active site differs and therefore the substrate in which it reacts with will also change. Enzymes are very specific and work to complete one task.
How Enzymes
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This links to the concentration differences between plant and animal cells. Catalase has a higher concentration within animal cells because the cell functions of an animal cell produce more hydrogen peroxide as a by-product and therefore more Catalase is required to convert the hydrogen peroxide.
The results recorded from trial three deviated from the trend shown in the first two trials. Reasons for this abnormality within the data could be explained by the fact that the tissues spent over 48 hours in the fridge. As a result, the particles have a tighter bond and therefore the energy level of the catalase differs. Overall in the animal cells, there were more bubbles produced in the third trial than either the first or second trial. This is because once taken out of the fridge and added to the hydrogen peroxide solution, an exothermic reaction occurs (heat released). This is justified because the bottom of the test tube was hot to touch. As highlighted by… as the temperature increases, the rate of the enzyme’s reaction speeds up. This links to the experimental results, the bubbles released from the third trial increased due to the
amplified
chemical reactions. Enzymes have a set of conditions at which they work perfectly; this is known
While these studies provide positive results the draw back to them are that the sample groups for each were considerably small which means that there is insufficient evidence to support
Without enzymes the existence of life is questionable since all metabolic processes in the cell cannot occur at a faster rate enough to sustain life. All the essential biological reactions in living things depend on enzymes’ catalytic activity. Enzymes are usually proteins, though some Ribonucleic Acid (RNA) molecules act as enzymes too, that speed up the rate of biological reactions without being consumed by the reaction. (Reece,2016, p.83) For instance, the presence of enzymes in the conversion of DNA to RNA allows a reaction that would otherwise take millions of years to occur in milliseconds. Like all catalysts, enzymes accelerate the rate of reaction by lowering the activation energy that is the amount of energy that must be put in for the reaction to begin. (Reece,2016, p.83)
Enzymes are able to catalyse due to their globular structure which has a region on their surface that has a specific shape. This shape is complementary to the shape of the reactant or reactants of the reaction that the enzyme will catalyse. This region on the enzyme is referred to as the active site which binds to the substrate in order for the reaction to occur.
Also, I will hold the test tube by the tip. If I were to grab the test tube with my whole hand, my body heat would be passed onto the test tube causing a temperature increase. Therefore, not creating a reliable set of results.
Enzyme Lab Conclusion In this lab three hypotheses were tested. If the catalase Solution is added then, the H2O2 will be broken down into harmless byproducts/ there will be an increase in temp compared to the control, if the boiled catalase is added to the H2O2, then there will be no significant increase in temp compared to the control, and if the catalase with acid is added to the H2O2, then there will also be no significant temperature increase compared to the control. The first hypothesis of, if the catalase solution is added to the H2O2, then there will be an increase in temp compared to the control, was proven correct. The control(the H2O2 with no catalase added) stayed at a constant temp of 23.5 degrees celsius throughout the 5 minutes
For the experiment, the enzyme Catalase was used at varied temperatures to measure oxygen produced by the breakdown of H2O2. Most enzymes are specialized molecules of protein that have a specific structure to them. They are catalysts in metabolic activity. Being that they are made from proteins, the shape comes from the folding or condensing of the long strands of amino acids. These polypeptides fold from primary, to a secondary, then tertiary structure.
Additionally, one of the participants (Alan) had exercised earlier in the day, possibly reducing muscle efficiency. This represents a systematic error since all of the trials done with Alan might have lower efficiency than expected. There was also one trial (70 bpm for participant 2, Matt) where the nose plug fell off in the
Consequently and in general, there is a noticeable difference between the analyses accorded to the fresh evidence
Enzymes Enzymes are vital in the survival of humans. There are thousands of specific enzymes in our bodies that are needed for chemical reactions to take place in our body. Enzymes are biological catalysts that help difficult reactions occur everywhere in life. An enzyme only reacts on certain substances in the range of certain temperatures and pH. The enzyme must be the right size and shape to fit the substrate which helps cause the reaction. If the enzymes environment changes (temperature and pH wise) the enzymes size and shape could change resulting in the substrate not fitting.
Each enzyme has specificity that is a result of the shape and types of R-groups involved at the active site (Freeman, 2014, p. 54).
One reason why observational studies and randomized trials yielded different results was observational studies cannot determine whether one event was the cause of another event. Observational studies are only capable of gathering information that may serve as evidence in preparing a hypothesis. They are not able to predict that one event is directly responsible for another event. In this article, researchers gathered information that since
Since the previous study did not show any significant results the researchers realized that since there was a longer period of time in postreview of course there was
With any study, there are limitations that are not counted for in the study, which may result unfavorably. Because we found no significance in all but one hypotheses, many factors may be contributed.
2012 the number of trials decreased by 26%, i.e. from 160 to 118 studies. The trough in these