Enzymes Lab Report

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Enzymes Lab Report Inroduction In this lab we explore an enzymes activity and how it can be affected by changes to its environment. An enzyme is a protein and is a catalyst to chemical reactions. It helps accelerate reactions by lowering the activation energy, which is needed for reactions in cells to progress at a higher rate. Activation energy is the minimum amount of energy needed for a chemical reaction to occur, yielding products from a given set of reactants. (Unit 7: Enzymes lab) Products are results of the an enzyme cleaving to a specific substrate, by means of an induced fit. The induced fit is located at the active site of the enzyme or region of the enzyme where the substrate is bound. The substrate is the reactant…show more content…
Then, three drops of milk were added to each tube and allowed to sit for 1 minute. Next, three drops of lactase were added to each tube and placed in the 40 degrees C water bath. They were left there for 10 minutes. After 10 minutes, a glucose strip was placed in each tube for one second, removed, and allowed to sit on the bench top for one minute. At the end of one minute the strip coloration was compared to the chart provided and the amount of glucose was determined in mg/dL. (Unit 7: Enzymes lab) Results Temperature experiment | |0 C | | |Glucose | | |(mg/dL) | |100 |250 | | | | |Control-Glucose (mg/dL) |EDTA-Glucose (mg/dL) | |Mean= |250.5 |94 | |Sample size= |98 |98 | |t= 50.1 df= 193 p < 0.0001 reject the null hypothesis Discussion Although lactase is a very effective catalyst to lactose metabolism, we see that it is very sensitive. As we expose it to change from it’s normal environment temperature and pH it became almost

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