Enzymology, Catalytic Mechanism, Carbohydrate Metabolism, Adenosine Triphosphate

In contrast, there are four metabolic stages happened in cellular respiration, which are the glycolysis, the citric acid cycle, and the oxidative phosphorylation. Glycolysis occurs in the cytoplasm, in which catabolism is begun by breaking down glucose into two molecules of pyruvate. Two molecules of ATP are produced too. Some of they either enter the citric acid cycle (Krebs cycle) or the electron transport chain, or go into lactic acid cycle if there is not enough oxygen, which produces lactic acid. The citric acid cycle occurs in the mitochondrial matrix, which completes the breakdown of glucose by oxidizing a derivative of pyruvate into carbon dioxide. The citric acid cycle produced some more ATPs and other molecules called NADPH and FADPH. After this, electrons are passed to the electron transport chain through

Enzymes speed up metabolic reactions necessary for life. Without them certain vital processes would not take place and the body would be unable to function.

The temperature can have a major impact on an enzyme. According to Campbell Biology author Reece etc. 2011 “The enzyme reaction will increase as the temperature increase with the increasing temperature….substrates collide with active sites more frequently when the molecules move rapidly.”(Reece etc 2011) Every enzyme hits its optimal temperature the reaction will be at its highest point.(Reece etc. 2011) When the

Temperature controls the speed the enzymes work at. Higher temperatures increase the kinetic energy which increases the chance of collision therefore speeding up the rate of

These results show how temperature of extreme high, or low affects enzyme activity. The highest rate of enzyme activity occurred at 37 Cº. Anything that was hotter or cold than 37 Cº slowed the reaction rate. As I thought, 100 degrees would denature the enzyme, and that was the case. The data provided shows exactly what temperatures enzymes work best, and worst. The objective was achieved as we discovered the different reaction rates under different temperatures. The results are reliable, as we know enzymes do not work well when under extreme heat or denaturation occurs. What I learned in this experiment was that enzymes don’t work well under cold temperatures because they tend to move slower. My hypothesis did not quite match, because I thought they work best at lower temperatures.

Most enzymes work best at body temperature, higher temps will cause the enzyme to no longer work properly

As stated before the three energy systems used by the body are the ATP-PC, anaerobic glycolysis and aerobic system. The ATP-PC and anaerobic glycolysis system (also known as lactic acid system) are anaerobicly based meaning that they don’t need a sufficient amount of oxygen to produce ATP. The aerobic system requires oxygen to produce ATP hence its name. All three system have fuels’ which produce energy. The ATP-PC uses phoso creatine and creatine phosphate, the lactic acid system uses glycogen and the aerobic system uses glycogen and triglycerides . Glycolysis refers to the breaking down of glycogen to from glucose which is used in ATP.

One of the most significant reactions in Glycolysis is reaction one which involves the phosphorylation of glucose to form glucose-6-phosphate. Through the transfer of the hydrolysis of ATP, this supplies energy for the reaction and makes it essentially irreversible, having a negative free energy change, which allows for a spontaneous reaction in cells. Although the preparatory phase is energy consuming and uses up 2 ATP, the pay off phase synthesizes 4 molecules of ATP, with the transfer of 4e- via 2 hydride ions to 2 molecules of NAD+. Therefore, a net gain of 2 ATP is achieved through the glycolytic pathway alone. Following the glycolytic pathway, due to the absence of oxygen, as oxygen cannot be supplied fast enough to undergo aerobic respiration, the athlete will instead, undergo lactic acid fermentation. Lactic acid fermentation involves pyruvate that is formed from the glycolytic pathway to be reduced to lactate, with the aid of the enzyme, lactate dehydrogenase, while the coenzyme Nicotinamide Adenine Dinucleotide (NADH) is oxidised to NAD+. The product NAD+ then re-enters the glycolytic pathway in order to produce 2 ATP. This process of lactic acid fermentation produces 2 ATP for each cycle, and thus, rapidly supplies the body with a small amount of energy. However, with the buildup of lactic acid in the body, the athlete will eventually encounter the feeling of discomfort as this accumulation of lactate causes the body to

Enzymes will denature if they get too hot or cold or if the pH of the solution they are in is too high or too

This is more low power/long duration and a prime example of this is a distance runner. This pathway requires oxygen to produce ATP, because carbohydrates and fats are only burned in the presence of oxygen. This pathway occurs in the mitochondria of the cell and is used for activities requiring sustained energy production. Aerobic glycolysis has a slow rate of ATP production and is predominantly utilized during longer-duration, lower-intensity activities after the Phosphogen and anaerobic systems have

Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology Department, 2011).

Enzymes are central to every biochemical process. Due to their high specificity they are capable of catalyzing hundreds of reactions that signifies their vast practical importance.

The lactic acid system is capable of releasing energy to resynthesise ATP without the involvement of oxygen and is called anaerobic glycolysis. Glycolysis (breakdown of carbohydrates) results in the formation of pyruvic acid

Removal of lactic acid in the glycolysis system is largely impacted by blood flow and heart rate.

Glucose for pyruvate is generated by glycogenolysis, which is a process breaking down glycogen to glucose. Glycogen is stored sugar in our muscles. Due to the buildup lactate muscles get tired and the anaerobic glycolysis last only for couple of minutes.