Escherichia Coli : An Ideal Model Organism

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Title : Studies of Large RNAP­NusG­70S Transcription­Translation Linking Complex Author : Dominique Gutierrez, Cristina Gutierrez­Vargas, and Dr. Joachim Frank Abstract Escherichia coli (E.coli) is an ideal model organism. Many mechanisms found in E.coli are resembled in other species. S10, a protein on the 30S subunit of the ribosome, is highly conserved and found in pathogens, including Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae. S10 has been shown to interact with NusG, a transcription elongation factor, linking the translational machinery to that of transcription. The coupling…show more content…
Introduction The relatively sudden rise in antibiotic resistance has resulted in the increasing pathogenicity of bacterial infection entailing the utilization of novel and more universal mechanisms in the development of new treatments. Both NusG and NusE/S10 are highlyconservedandplayrolesintranscriptionandtranslation(Werner,2012).Sinceits identification as an E.coli transcription elongation factor involved in N protein phage lambda transcriptional antitermination in 1992 (Li J., et.al), N­utilization substance G, NusG a 20.5 kDa protein has been suggested toactasadirectphysicallinkercoupling transcription and translation (Burmann et al., 2010). Through NMR and biochemical assay studies Mooney (2009) found that NusG has two domains connected by a flexible linker that distinctly makeupthecharacteristic functionalities of NusG. The N­TerminalDomain,NTDcomposedof3alphahelicesand 4 beta strands that form an antiparallel sheet has a hydrophobic pocket by which it interacts and binds to the β′ clamp helices of RNA Polymerase, RNAP. Through this
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