Escherichia Colo Transformation

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In the following experiment, Escherichia coli were transformed using six separate agar plates. Transformation occurs when plasmid DNA is uptake into the bacterial cell. The two ways transformation is facilitated is by placing them in calcium chloride (CaCl2) and heat shock. These two methods allowed the bacterial cells to become competent or more open to the uptake of plasmid DNA. The purpose of this experiment was to transform bacterial cells with plasmid containing ampicillin resistance and lux genes. It was hypothesized that the lux genes would have created a light-emitting reaction on the bacteria while the ampicillin genes should have made the bacteria resistant to ampicillin attacks and be able to grow normally. The experiment results
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