Essay On Genome Editing

Humans have been genetically engineering organisms for nearly 10,000 years using traditional methods of modification—among these methods include selective breeding and crossbreeding. Though effective, these methods were unreliable and were only able to change certain traits. A lack of control over our genetic material proved to be a clear hindrance to our species; when harnessed, advancements in other fields of knowledge would be immeasurable. Once seen as an impossible task, scientists have been able to exploit genes and take control of them. CRISPR-Cas9 is a system that allows scientists to cleave off sections of DNA and artificially modify them by inserting a mutation into the place of the old DNA. This is exceptionally precise, whilst

The Cas9 protein is an enzyme that effectively acts as a pair of molecular ‘scissors’, cutting

With modern technology comes the breakthrough of the decade by altering the human genes. This altering gene invention is called CRISPR/Cas9. However, this invention in the beginning stages of altering genes, began with rats until perfection. The process began early with the embryo stages to edit the genes. With the introduction of CRISPR surrounds a lot of controversy. Some people believe editing genes is playing with the hands of God and refuse to believe in CRISPR. With the article, “Let’s Hit Pause Before Altering Humankind”, by David Baltimore believes CRISPR is a tool with no good intentions. With this information the article should not be published with being against CRISPR.

A defective gene is most times caused by a virus getting into your DNA which later turns into a disease. The way CRISPR can fix this disease is going into your DNA, finding the

Human gene editing has long been controversial topic; however, precise techniques that accomplish this feat have only recently been discovered. According to the Welcome Genome Campus in the UK, the most versatile and simplest technique, called CRISPR-Cas9, allows scientists to cut, alter, or add to sections of the DNA sequence of living organisms (“What Is CRISPR-Cas9?”). This astonishing technology has nearly endless applications, including the potential to eradicate genetic diseases in humans that currently have no cure. This could have vast implications for people who suffer with disease and the economy of the region in which they live, but the technology has yet to be commercialized. The

In “Life the Remix,” Alice Park discusses the impact and influence CRISPR has on science as well as its potential and risks. CRISPR—“clustered regularly interspaced short palindromic repeats”—is a technique to alter DNA, virtually for anything involving DNA. Although there have been attempts to edit DNA, none were as cheap and simple as CRISPR. This technique, which is based on the immune system of a bacetria, revolutionizes genetics after the subsequent discoveries of the molecular scissors enzyme: Cas9 and a method to efficiently and accurately edit human DNA using CRISPR, explains Park.

Every few years, advancements in technology alter the way scientists do their work. Recently CRISPR-Cas9, a RNA useful for working organisms in the animal kingdom has proven itself beneficial on a gene-editing platform. After performing many abortive attempts to manipulate gene function, including homologous recombination and RNA interference, scientists have finally had a breakthrough with CRISPR-Cas9.

CRISPR/Cas9 DNA editing system is a prokaryotic immune system that becomes resistant to foreign genetics such as plasmids. CRISPR stands for Clustered Regularly-Interspaced Short Palindromic Repeats, which are prokaryotic DNA strands that have a short repetition base sequence. Cas9 is a nuclease enzyme that is used to cut into DNA strands that are associated with CRISPR type II. CRISPR/Cas9 system has several advantages when using this technology in the biological and biomedical sciences. It also has a few disadvantages that cause concerns when using this system in the medicine field. However, before all of this can be discussed, a depth of what CRISPR/Cas9 is and where it comes from and why is it important to the biology field.

Even though it was discovered in 1987 by a research team at Osaka University, the true power of CRISPR, which functions as an adaptive bacteria immune defense, was not realized until the completion of the Human Genome Project in the early 2000’s (Carroll and Zhou 63-64). There are only two components of CRISPR, a guide RNA sequence (gRNA) and a Cas9 endonuclease protein. When the gRNA binds to its matching template DNA sequence, the Cas9 protein cuts the template DNA sequence resulting in the inactivation of that specific gene (Hille and Charpentier 3). In addition, a mutant gene can be replaced by adding another piece of DNA with the desired sequence, which will bind to the cut DNA template strand and become incorporated into the host

The US and Korean team created 58 human embryos while using the CRISPR-Cas9 they were able to repair faulty genes up to 70%. (…)

CRISPR is versatile in that any target sequence can be modified by simply altering the gRNA sequence. In addition, multiple genes can be edited at the same time with great specificity (Cong 88-89). The convenience and accessibility of CRISPR resourced have also allowed thousands of laboratories worldwide to study CRISPR in different ways, which has broadened the horizons of its biomedical and clinical implications (Collins et al 259). Overall, the ease and simplicity of CRISPR technology has allowed for a rapid increase in the understanding of genome editing, which will allow CRISPR to revolutionize how certain conditions will be treated.

This is done by inserting a break in the DNA and tricking a cells natural DNA repair mechanism into introducing the change one wants. In 2012 two studies were conducted by separate groups, to help transform bacterial Crisper-cas9 into a simple, programmable editing tool. The studies concluded that cas9 could cut any region of DNA, by simply changing the sequence of crRNA, which binds to a complementary DNA target. This system was further simplified when Martin Jinek and his colleagues fused crRNA and tracrRNA to create a single guide RNA. Therefore making it so gene editing only requires two components: a guide RNA and the cas9 protein (Aparna Vidyasagar,

CRISPR-Cas9 is a gene editing tool that cuts out, replaces, and alters unwanted pieces of DNA. This process starts by scientists creating a synthetic RNA guide with a specific sequence. Upon entering the cell, the RNA guide meets up with the enzyme Cas9. The RNA then leads the Cas9 to a predetermined section of DNA. The Cas9 unzips the DNA which allows the RNA

Genetically modifying the human genome is a topic that has been discussed for several decades, but just recently it is becoming a reality. Through many advancements of technology, the CRISPR method has emerged, and it is providing the opportunity for genetically modifying the human genome to take off. However, allowing the use of the CRISPR method is not just a black and white decision. Modifying the human genome is a hot debate in the science and medical fields todays. Arguments in support of modifying the human genome including the following; offers the opportunity to cure genetic diseases, improves the value of life, it is similar to any other intrusive medical therapy, and it provides beneficence. Nevertheless, although we have this type of technology at our fingertips, there are many concerns with editing the human genome. Some of the concerns include the following; the potential safety issues, the unknown consequences that may evolve from a procedure of this kind, sacrificing human dignity, and finally nonmaleficence. There are arguments in support of modifying the human genome to cure genetic diseases, and there are arguments in opposition of modifying the human genome to cure genetic diseases because of the ethicality of the procedure.

In the recent years there has been a new technology created, called CRISPR-Cas, that allows for gene-editing in germline cells to potentially treat or prevent genetic diseases and conditions. There has already been uses of this technology in clinical trials to edit immune cells that treat cancer. While this sounds like something that would be a fantastic idea it is very controversial and raises concerns of whether it is ethical or not. As of right now, we should be able to edit our cells, but only somatic cell and not our germ line cells.