Essay On Mitochondrial Trafficking

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1. Discussion In this study, mitochondrial trafficking velocity, distance travelled, abundance and localization were all compared between WT and RTT neurons. Furthermore, the effects on mitochondrial trafficking velocity and distance travelled of SW-100 were also examined. Observations showed no significant difference in the number of mitochondria/µm2 around the soma or in the ratio of moving mitochondria between WT and RTT neurons. In addition, there was no significant difference in the distance travelled by mitochondria between WT and RTT mitochondria, however, WT mitochondria travelled significantly faster than RTT mitochondria. Mitochondrial trafficking velocity was not to be significantly affected by 1 µM of SW-100 in either…show more content…
Thus, these results support part of the first hypothesis. The observations showed that WT and RTT mitochondria do not travel to significantly different distances. These findings suggest that mitochondria of both genotypes eventually reach the same end point, which can be assumed to be the synapse. This is further supported by the fact that there are comparable numbers of mitochondria distributed at different distances from the soma in WT and RTT neurons. Therefore, WT and RTT mitochondria do reach the synapse, but do so at different velocities. Since it takes longer for the RTT mitochondria to reach the synapses, it means that they would have synthesized more ATP by the time they reach the synapse. This may help explain the cause of synaptic dysfunction in RTT neurons, as the increased demand on ATP for trafficking will reduce their efficiency in the synapse. The results showed that there was no significant difference in mitochondrial velocity in neurons of both genotypes, between the SW-100 and placebo treated groups. This contradict part of the first hypothesis. Furthermore, the distance travelled by mitochondria of both genotypes was significantly larger in the placebo group than the SW-100 group. This suggests the 1 µM SW-100 does not enhance mitochondrial trafficking velocity, but inhibits the distance mitochondria can travel to. The success of the macros was evident through their ability to conduct the entirety of the analysis
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