Ethos Synthesis Report

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An ethos reflux microwave extractor (1000 W, Frequency 2450 MHz, Milestone, Italy) was used for the extraction. The extraction process was done in an enclosed vessels and no evaporation was observed. 10 g of homogeneous powdered Vernonia amygdalina leaf samples was mixed with a measured quantity of extracting solvent (distilled water or ethanol). The mixture was loaded into the microwave extractor and the parameters were set. After the extraction process, the mixture was cooled to room temperature and filtered. The filtrate was then dried using a rotary evaporator and stored in a 4 ºC prior to further
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