Eukaryotic Chemotaxis Lab Report

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Delving into the Process of Eukaryotic Chemotaxis The mechanism in which cells move either towards, or away from a stimulus is referred to as chemotaxis (1). Chemotaxis is initiated in eukaryotic cells when the cells first sense an external chemotactic gradient (2). The presence of this gradient is sensed by a class of receptors, called 7-transmembrane heterotrimeric G-protein coupled receptors (GPCRs) (2). As the name implies, these proteins are located in the plasma membrane of eukaryotic cells. This external gradient triggers an intracellular Phosphatidylinositol (3,4,5)-trisphosphate (PIP3) gradient to form (2). PIP3 is a phospholipid that is located in the plasma membrane of eukaryotic cells (3). This PIP3 gradient induces a signaling…show more content…
Leukotrienes use lipid signaling to aid in autocrine signaling and paracrine signaling to regulate immune responses (10). Leukotrienes (LTs) bind to a family of GPCRs called leukotriene receptors (10). The binding of LTs to their receptors causes a conformational change in the receptor that triggers the lipid-signaling pathway, which in turn, stimulates proinflammatory activities such as endothelial cell adherence and chemokine production (10). Leukotrienes are known to induce asthma by reducing the airflow of the alveoli (11). However production of leukotrienes can be reduced by ingesting dietary omega-3 fatty acids…show more content…
Motility is present even in the absence of a stimulus, if the organism contains flagella or pseudopodia (14). However, if the cells are exposed to a gradient of chemoattractant or chemorepellent, their motility is biased toward or away from higher concentrations of that stimulus (14). When Dictyostelium are exposed to various cAMP concentrations, more pseudopodia are formed and are extended towards the areas of high cAMP concentrations (14). This process is mirrored by neutrophils, which extend pseudopodia rhythmically towards positive chemotactic stimuli
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