Evaluation Of The Effects Of Lactobacilli Containing Probiotics

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Evaluation of the Effects of Lactobacilli Containing Probiotics in Non-Surgical Treatment of Chronic Periodontitis: A Clinical and Microbiological Study
Reham Lotfy Aggour
Lecturer of Oral Medicine, Periodontology, Oral Diagnosis and Oral Radiology, Faculty of Dentistry, October 6 University

Background & Objectives: There is limited information on the effects of beneficial bacteria (probiotics) on management of periodontal disease. The purpose of the present study was to investigate the clinical and microbiological effects of Lactobacilli containing probiotic sachets as an adjunct to non-surgical periodontal therapy.
Methods: Thirty chronic periodontitis patients, who satisfied the inclusion and ex¬clusion criteria, were
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Each group comprised 15 patients. After oral hygiene instructions and full mouth SRP, all patients resumed mechanical tooth cleaning twice daily using a soft tooth brush and the Bass technique. Patients in the test group were instructed to administer the probiotic sachets after brushing.
Clinical parameters
The following clinical measurements were performed on days 0 (baseline; BL), 1 month and 6 months post treatment using Williams graduated periodontal probe at six sites (distal, mid and mesial aspects for both buccal and lingual sites) of each tooth: probing pocket depth (PPD) and clinical attachment loss (CAL) excluding the third molars. Plaque index (PI) (12) and full mouth bleeding index (FMBI) (13) were measured by calculating the percentage of sites that revealed the presence of bleeding on baseline, 1 month and 6 months post treatment. An examiner who was masked with respect to the experimental procedures carried out all measurements of clinical parameters.
Microbiological procedures:
After removal of supragingival dental plaque, the areas corresponding to the deepest pocket of each patient 's dentition quadrant were washed with water spray, isolated with cotton rolls, and dried. A sterile paper point # 30 was inserted into the bottom of the periodontal pocket for 30 seconds then placed in sterile Eppendorf tubes containing PBS (phosphate buffer solution, pH 7.4) transport medium, and kept at -4° C until

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